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Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf-?? activation in blunt pulmonary contusion model
(Turkish Assoc Trauma Emergency Surgery, 2019) Karaboğa, İhsan
BACKGROUND: Pulmonary contusion (PC) is an important life-threatening clinical condition characterized by lung injury and inflammation. Caffeic acid phenethyl ester (CAPE) is a biological agent with potent antioxidant and anti-inflammatory effects. This study aimed to investigate the potential effects of CAPE on tissue damage, nuclear factor kappa-beta (Nf-kappa beta) activity, inducible nitric oxide synthase (iNOS) synthesis, and pulmonary apoptosis in an experimental PC model. METHODS: Forty adult Wistar albino rats were used in this study and divided into four groups as follows: control, PC, PC + CAPE, and CAPE. CAPE was administered intraperitoneally for seven days following PC formation (10 jimol/kg, dissolved in dimethyl sulfoxide). Wet/dry weight ratio in lung tissue was determined. The pulmonary tissue was examined using hematoxylin-eosin and Masson's trichrome histochemical staining and also by scanning electron microscopy. Nf-kappa beta and iNOS activities in the lungs were determined by the indirect immunohistochemical method. Pulmonary apoptosis was detected by the TUNEL method. RESULTS: Increased leukocyte infiltration score, pulmonary edema, alveolar damage, and increased Nf-kappa beta and iNOS activities were determined in the PC group. CAPE administration inhibited Nf-kappa beta and iNOS activities and pulmonary apoptosis. CONCLUSION: In this study, the findings showed that CAPE inhibited tissue damage by suppressing inflammatory mediators of Nf-kappa beta and iNOS activities. Also, CAPE was found to be protective in the lung tissue and could be used as a therapeutic agent.
Cardioprotective effect of caffeic acid phenethyl ester on cardiac contusion following blunt chest trauma in rats
(Taylor and Francis Ltd, 2019) Bıçakçı, N.; Karaboğa, İhsan; Dökmeci, Ayşe Handan; Güzel, S.; Fidanol Erboğa, Z.
We investigated the effects of caffeic acid phenethyl ester (CAPE) on cardiac damage after blunt chest injury. Forty male adult Wistar albino rats were divided into four groups; control, cardiac contusion, cardiac contusion + CAPE, and CAPE. CAPE, 10 mmol/kg, was administered intraperitoneally for 7 days following cardiac contusion. Heart tissue and blood were obtained at the end of the experimental period. Cardiac histopathology was determined using hematoxylin & eosin (H & E) staining. Expression of tumor necrosis factor-alpha (TNF-?) in cardiomyocytes was determined using immunohistochemistry. Cardiac apoptosis was determined using the TUNEL method. Serum creatine kinase (CK), creatine kinase-muscle/brain (CK-MB) and lactate dehydrogenase (LDH) levels were determined using spectrophotometric methods. The serum cardiac troponin I (C-TI) level was measured using the ELISA method. Myofibril loss was detected in the cardiomyocytes of the cardiac contusion group. Increased apoptosis and TNF-? expression were observed in the cardiac contusion group compared to the control group. Increased CK, CK-MB, LDH and C-TI levels were found in the cardiac contusion group. We found that CAPE administration improved myocardial function. Compared to the cardiac contusion group, CK, CK-MB, LDH and C-TI levels decreased significantly in the cardiac contusion + CAPE group. Administration of CAPE significantly inhibited apoptosis and cardiac TNF-? expression. Our findings demonstrate the therapeutic effects of CAPE for cardiac contusion damage after blunt chest trauma. © 2019, © 2019 The Biological Stain Commission.
Ebselen, an Active Seleno-Organic Compound, Alleviates Articular Cartilage Degeneration in a Rat Model of Knee Osteoarthritis
(Springernature, 2022) Okuyan, Hamza Malik; Yurtal, Ziya; Karaboğa, İhsan; Kaçmaz, Filiz; Kalacı, Aydıner
Osteoarthritis (OA) is a prevalent articular disease mainly characterized by extracellular matrix degradation, apoptosis, and inflammation, which lead to cartilage destruction and abnormal bone metabolism. With undesirable side effects, current limited symptomatic treatments are aimed at relieving pain and improving joint mobility in patients with OA. Intra-articular (IA) hyaluronic acid (HA) injection, as a nonsurgical therapy, is commonly used in the clinical management of knee OA, but the efficacy of this therapeutic option remains controversial. Ebselen has tremendous pharmacological importance for some diseases due to its antioxidant, antiapoptotic, and anti-inflammatory features. However, there is no research examining the therapeutic effect of Ebselen in OA using the rat OA model. Therefore, we aimed to investigate the therapeutic effect of Ebselen on cartilage degeneration and its role in bone morphogenetic protein 2 (BMP2) and nuclear factor kappa B (NF-kappa B) signaling in the molecular pathogenesis of OA. We induced a knee OA model in rats with an IA injection of monosodiumiodoacetate (MIA). After the treatment of Ebselen, we evaluated its chondroprotective effects by morphological, histopathological, and immunohistochemical methods and an enzyme-linked immunosorbent assay. We report for the first time that Ebselen treatment alleviated articular cartilage degeneration in the rat knee OA model and reduced MIA-induced BMP2 and NF-kappa B expressions. In addition, our results unveiled that Ebselen decreased IL-beta and IL-6 levels but did not affect COMP levels in the rat serum. Ebselen could be a promising therapeutic drug for the prevention and treatment of OA by alleviating cartilage degeneration and regulating BMP2 and NF-kappa B expressions.
Effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid-induced experimental colitis model
(Cellular and Molecular Biology Association, 2018) Polat, Fatin Rüştü; Karaboğa, İhsan; Polat, Muhammed Semih; Erboğa, Zeynep Fidanol; Yılmaz, Ahsen; Güzel, Savaş
In our study, the effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model was investigated through different methods. Eighteen Wistar albino male rats were divided in to three groups: Group I (Control, n = 8; 1 ml physiological saline), Group II (Colitis, n = 8; 1 ml TNBS), Group III (Hesperetin, n = 8; 1 ml TNBS and 100 mg/kg hesperetin). Macroscopic and microscopic scores were calculated to determine the damage to the colon at the end of the experiment. Serum tumor necrosis factor-? (TNF-?) and tissue interleukin-6 (IL-6) levels were determined using the ELISA method. Myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were investigated spectrophotometrically. The TUNEL method was used for the detection of apoptotic cells in the colon tissue. Inducible nitric oxide synthase (iNOS) and nuclear factor-kappa-B (NF-??) expression in the colon were determined immunohistochemically. Hesperetin administration has shown to significantly reduce levels of MPO, MDA, and proinflammatory agents (TNF-?, IL-6, and NF-??). It has also been proven to inhibit mucosal apoptosis. This study indicates that hesperetin is protective against TNBS-induced colitis model via antiinflammatory, antioxidant and antiapoptotic effects. © 2018 by the C.M.B. Association. All rights reserved.
Effect of hesperetin on systemic inflammation and hepatic injury after blunt chest trauma in rats
(Taylor and Francis Ltd, 2020) Duran, Y.; Karaboğa, İhsan
We investigated the protective effect of hesperetin on hepatic damage after blunt chest trauma in rats using histological and biochemical methods. We used 18 adult male rats in three groups of six: control, chest trauma and chest trauma + hesperetin. Chest trauma was caused by dropping a metal cylinder onto the right hemithorax. Hesperetin, 100 mg/kg, was administered orally for 7 days. At the end of the seventh day, liver tissue samples were obtained. Serum tumor necrosis factor-alpha (TNF-?), interleukin 1-beta (IL-1?), alanine aminotransferase (AST), aspartate transferase (ALT) and lactate dehydrogenase (LDH) enzyme activities were measured in blood samples taken from the heart. The general structure of liver tissue was investigated using hematoxylin and eosin staining. Nuclear factor kappa beta (Nf-??) expression in liver tissue was determined by the indirect immunohistochemical method. Apoptosis was determined using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) method. Decreased TNF-?, AST and ALT enzyme activity, fewer histopathological changes and lower Nf-kB expression were observed in the hesperetin treated group compared to the chest trauma group. We also found reduced hepatic apoptosis in the chest trauma + hesperetin group compared to the chest trauma group. Hesperetine inhibits liver damage by reducing proinflammatory cytokines and by suppressing Nf-?? activity in a blunt chest trauma model in rats. © 2019, © 2019 The Biological Stain Commission.
Etanol Uyarımlı Sıçan Akut Mide Mukoza Hasar Modelinde Hypericum Perforatum'un Koruyucu Etkilerinin İncelenmesi
(Namık Kemal Üniversitesi, 2017) Karaboğa, İhsan; Dökmeci, Ayşe Handan; Ovalı, Mehmet Akif; Yılmaz, Ahsen
Bu çalışma sıçanlarda absolü alkol ile oluşturulan gastrik ülserde H. perforatum'un antiapoptotik ve antiinflamatuvar etkilerini değerlendirmek amacıyla yapılmıştır.
Gastroprotective effect of apricot kernel oil in ethanol-induced gastric mucosal injury in rats
(Taylor & Francis Ltd, 2018) Karaboğa, İhsan; Ovalı, M. A.; Yılmaz, A.; Alpaslan, Mehmet
We investigated the gastroprotective effect of apricot kernel oil on ethanol induced gastric ulcer in rats. Male Wistar albino rats were divided into control, ethanol and apricot kernel oil + ethanol groups. The fatty acid composition of apricot kernel oil was determined using GC-MS. A gastric ulcer index was defined as the area percentage of the gastric mucosa consisting of ulcerated tissue. Gastric tissue was investigated by TUNEL staining for apoptosis, immunohistochemical iNOS staining, measurement of gastric IL-10 and IL-6 expression by ELISA and assays of catalase, malondialdehyde and superoxide dismutase. The ethanol group exhibited a higher gastric ulcer score, increased IL-6 level, increased number of inducible nitric oxide synthase-positive and TUNEL positive cells, and a higher MDA level compared to the control group. The apricot kernel oil + ethanol group exhibited significantly fewer gastric lesions compared to the ethanol group. Apricot kernel oil protects rat gastric mucosa against ethanol induced injury by its anti-inflammatory, anti-oxidative and anti-apoptotic effects, and might be useful for reducing the severity of gastric ulcers.
Humic Acid Has Protective Effect on Gastric Ulcer by Alleviating Inflammation in Rats
(Pleiades journals, 2022) Şehitoğlu, Müşerref Hilal; Öztopuz, Ö.; Karaboğa, İhsan; Ovalı, M. A.; Uzun, Metin
The new agents are needed in treatment of gastric ulcer that have less side effects, adequate efficacy, and no drug interactions. In this study, we aimed to investigate the potential protective effects of humic acid on experimental gastric ulcer. Wistar Albino male rats (n = 48) were randomly divided into 8 groups as follow; Control (without any applications), Humic acid (50 mg/kg), ethanol group (1 mL/rat), and indomethacin group (25 mg/kg). In the treatment groups, both gastric ulcer model and humic acid 50 mg/kg were applied. In addition, famotidine the antiulcer drug was used as positive control. All medications were administered by oral gavage. Levels of ADAM10 and ADAMTS12 in gastric mucosa were determined by ELISA method. Hematoxylin-Eosin (H&E) staining, iNOS, and PCNA immunohistochemical staining were performed for histopathological investigations. Apoptosis was demonstrated by using the TUNEL method. In addition, the levels of inflammatory cytokines (TNF-?, IL-6, IL-10) and caspase-3 gene were determined by qRT-PCR. ADAM10 and ADAMTS12 levels significantly increased in the treatment groups compared to the ulcer groups (p < 0.05). The experimental groups showed mucosal erosion, bleeding, leukocyte infiltration and edema. Treatment with humic acid and famotidine was found to suppress iNOS activity, thereby decreasing proinflammatory activity and preventing damage to the gastric mucosa, while reducing the number of apoptotic cells. IL-6, IL-10, TNF-? and caspase-3 levels were significantly decreased in the treatment groups compared to damaged gastric mucosa. As a result, humic acid may be defined as a potential protective agent with its anti-inflammatory effect in gastric ulcer. © 2022, Allerton Press, Inc.
Immunohistochemical examination of anti-inflammatory and anti-apoptotic effects of hesperetin on trinitrobenzene sulfonic acid induced colitis in rats
(Taylor & Francis Ltd, 2019) Polat, Fatin Rüştü; Karaboğa, İhsan
The trinitrobenzene sulfonic acid (TNBS) induced colitis model is used to investigate the pathogenesis of ulcerative colitis. Colon inflammation and apoptosis are associated with tissue damage in ulcerative colitis. Hesperetin is a natural flavonoid that exhibits antioxidative, anti-inflammatory and anti-apoptotic properties. We investigated the effects of hesperetin on tumor necrosis factor-alpha (TNF-), protein tyrosine phosphatase, receptor type C (CD45), caspase-3 and Bax expressions in TNBS in induced colitis model in rats. Male rats were divided into three groups: control group treated with 1ml physiological saline, colitis group, and colitis +hesperetin group treated with TNBS and hesperetin. Hesperetin treatment was applied for 10days starting 3days prior to colitis induction. At the end of the experiment, TNF-, CD45, caspase-3 and Bax expressions in colon tissue were determined using indirect immunohistochemistry. Increased immunoreactivity of both inflammation markers, TNF-, CD45, and apoptotic markers, caspase-3 and Bax, was detected in the colitis group. Hesperetin treatment effected significant reduction of all parameters. Hesperetin treatment prevents colon damage owing to its anti-inflammatory and anti-apoptotic effects.
In vivo protective effects of upper zone of growth plate and cartilage matrix associated protein against cartilage degeneration in a monosodium iodoacetate induced osteoarthritis model
(Canadian Science Publishing, 2020) Okuyan, H.M.; Terzi, M.Y.; Karaboğa, İhsan; Doğan, S.; Kalacı, A.
Osteoarthritis (OA) is a degenerative disease affecting the majority of over 65 year old people and characterized by cartilage degeneration, subchondral abnormal changes, and inflammation. Despite the enormous socioeconomic burden caused by OA, currently, there is no effective therapy against it. Upper zone of growth plate and cartilage matrix associated protein (UCMA) is a vitamin K dependent protein and has a critical role in pathophysiological conditions associated with bone and cartilage. However, there is no research on the protective role of intra-articular UCMA treatment in OA pathogenesis. Therefore, we aimed to investigate the potential therapeutic role of UCMA in an in vivo model of OA. We report for the first time that intra-articular UCMA injection ameliorated cartilage degeneration in a monosodium iodoacetate induced OA rat model. Furthermore, the OA-induced activation of nuclear factor kappa B and bone morphogenetic protein 2 signals was attenuated by UCMA. Our results indicated that UCMA decreased cartilage oligomeric matrix protein levels but did not affect interleukin 6, total antioxidant status, and total oxidant status levels in the serum. In conclusion, UCMA exhibited a therapeutic potential in the treatment of OA. This protective effect of UCMA is possibly achieved by reducing the aggrecanase activity and the production of inflammatory cytokines. © 2020, Canadian Science Publishing. All rights reserved.
Investigation of Protective Effect of Hypericum Perforatum on Ethanol-induced Acute Gastric Mucosal Injury in Rats
(Tekirdağ Namık Kemal Üniversitesi, 2017) Karaboğa, İhsan; Dökmeci, Ayşe Handan; Ovalı, Mehmet Akif; Yılmaz, Ahsen
Objective: This study was conducted to evaluate the antiapoptototic and antiinflammatory effect of Hypericum perforatum in the rats induced gastric ulcer by absolute ethanol. Materials and Methods: Forty male Wistar albino rats were used in this study. Rats were divided four group, randomly. Group I (Control, n=10), Group II (Ethanol, n=10, 1 mL ethanol, orally, 90 min) Group III (H. perforatum + ethanol, 1 mL H. perforatum, 120 min; 1 mL ethanol, 90 min, orally), Group IV (Olive oil + ethanol, 1 mL olive oil, 120 min; 1 mL ethanol, 90 min, orally). Gastric ulcer score was determined in gastric mucosa by morphometrically. Histopatological staining, Immunohistochemical iNOS and PCNA staining, apoptotic TUNEL staining, gastric IL-10 and IL-6 expression (ELISA) and SOD, MDA, CAT levels were assessed in gastric tissue. Results: The results showed that ethanol incuded gastric damage, higher gastric ulcer index score, increased proinflammatory IL-6 level, elevated number of iNOS and TUNEL-positive stained cell number as well as higher MDA level in the group II (ethanol). Pre-treatment of H. perforatum and olive oil (group III and IV) significantly attenuated the gastric lesions as compared to the group II (ethanol). Conclusion: It was concluded that H. perforatum and olive oil may represents a potential therapeutic option to reduce the risk of gastric mucosal ulceration.
Investigation of the protective effect of erdosteine against cyclosporine-induced injury in rat liver with histological and biochemical methods
(Tubitak Scientific & Technical Research Council Turkey, 2015) Nacar, Ahmet; Karaboğa, İhsan; Okuyan, H.M.; Kaplan Sefil, Nebihat; Nacar, Emel; Motor, Sedat; Özkan, Orhan Veli
Background/aim: In the present study, the protective effect of erdosteine against cyclosporine-induced injury in rat liver was investigated with histological and biochemical methods. Materials and methods: Thirty-two Wistar albino male rats were randomly divided into 4 groups: control (n = 8), cyclosporine (n = 8, 20 mg kg(-1) day(-1) i.p.), cyclosporine + erdosteine (n = 8, erdosteine 12 mg kg(-1) day(-1) orally), and erdosteine (n = 8). At the end of day 12, liver tissues were removed for histological and biochemical analysis. After liver tissues were fixed in 10% buffered neutral formalin, routine histological processes were applied and tissue sections were stained with hematoxylin and eosin, periodic acid-Schiff, and elastic fiber stain methods. One hundred lobules of liver were examined for each group and evaluated statistically. The levels of malondialdehyde and glutathione peroxidase, as well as the activities of superoxide dismutase, were determined. Results: The cyclosporine group showed significant histopathological changes compared to the control. In the cyclosporine + erdosteine group, histopathological changes of hepatic damage were markedly reduced. Histological findings were supported by biochemical results. Conclusion: Erdosteine could attenuate cyclosporine-induced liver injury.
Investigation of the relationship between the Th17/IL-23 pathway and innate-adaptive immune system in TNBS-induced colitis in rats
(Mashhad Univ Med Sciences, 2017) Karaboğa, İhsan; Demirtaş, Selim; Karaca, Turan
Objective(s): This study was aimed at investigating immune activations of the 2,4,6-trinitrobenzene sulfonic acid(TNBS)-induced colitis model in colonic mucosa by immunohistochemical and Western blot methods. Materials and Methods: For this purpose, 16 female Wistar albino rats were divided into two random groups of control (n=8) and colitis (n=8). The experimental colitis model was induced by intracolonicadministration of TNBS (25 mg/rat). Control animals received only rectal saline for the same time. The animals were sacrificed on the 15th day after TNBS administration, and colon tissue was removed and examined morphologically. Colon samples were stained immunohistochemically with anti-CD3, anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-CD45, anti-TNF-alpha, anti-IL-17, anti-IL-22 and anti-IL-23 antibodies. Additionally, the colonic tissue IL-17 and IL-22 expressions were examined by the Western blot method. Results: In the experimental results, it was determined that there was a significant decrease in body weight and an increase in colon weight in the colitis group when comparing initial experiments. The colon tissue ulcerations, inflammation, crypt loss and Goblet cell loss were observed in the colitis group in microscopic examinations. The immunohistochemical positive cell numbers significantly increased in the colitis group. The immunoreactive lymphocytes in the propria, intracryptal and submucosal layers were found to be increased in the colitis group of rats. In addition, IL-17 and IL-23 expressions were increased in colitis colon mucosa found by Western blot analysis. Conclusion: The Th17/IL-23 pathway and IL-22 serve important roles in the pathogenesis of ulcerative colitis, and will be further examined by study.
Kafeik Asit Fenetil Ester Sıçanlarda Göğüs Travması Sonrası Gelişen Hepatik Hasarı Nükleer Faktör Kappa Beta ve İndüklenebilir Nitrik Oksit Sentezini Baskılayarak Azaltır
(2019) Karaboğa, İhsan
Amaç: Bu çalışmada göğüs travması modeli oluşturulansıçanlarda meydana gelen hepatik hasara karşı kafeik asitfenetil ester (KAFE)' nin Nükleer Faktör Kappa Beta (Nf-??)ve indüklenebilir nitrik oksit sentaz (iNOS) aktivitesi üzerineetkisinin incelenmesi amaçlandı.Yöntem: Çalışmada 40 adet erişkin Wistar albino erkek sıçansırasıyla 4 gruba ayrıldı; kontrol, göğüs travma modeli, göğüstravma modeli+KAFE ve KAFE grubu. KAFE uygulaması 7gün boyunca 10 µmol/kg/gün dozda intraperitoneal olarakgerçekleştirildi. Deney süresi sonunda karaciğer dokusualınarak meydana gelen histopatolojik değişikliklerHematoksilen-Eozin (H&E) boyaması ile değerlendirildi.Ayrıca hepatik Nf-?? ve iNOS aktiviteleri indirekimmünohistokimyasal yöntemle incelendi.Bulgular: Göğüs travması uygulanan grupta hepatositkordonlarında dejenerasyonları ve sinüzoidal dilatasyonbulgularına rastlandı. Göğüs travması+KAFE grubundahistopatolojik bulguların göğüs travması grubuna göre dahahafif seyrettiği belirlendi. Ayrıca, göğüs travması+KAFEgrubunda, göğüs travması grubuna kıyasla hepatik Nf-?? veiNOS immünreaktivitesinde istatistiksel olarak anlamlıderecede bir azalma olduğu görüldü (p<0.05).Sonuç: KAFE' nin göğüs travması uyarımlı sıçan hepatikhasarında Nf-?? ve iNOS aktivitesini baskılayarak koruyucuetki gösterdiği belirlendi.
Natriüretik Peptitler
(Namık Kemal Üniversitesi, Tıp Fakültesi, 2014) Demirtaş, Selim; Karaboğa, İhsan; Karaca, Turan
Natriüretik peptitler; atrial natriüretik peptit (ANP), B-tipi natriüretik peptit (BNP), C-tipi natriüretik peptit (CNP), Dtipi natriüretik peptit (DNP) nörohormonlarından oluşur. Bu hormonlar kalp, beyin, endotel ve diğer organlardan salınmaktadır. Natriüretik peptitler yaygın etki göstermektedirler. Natriürez, diürez, kan hacmi, kan basıncı, yağ metabolizması, kemik büyümesi ve hücre çoğalmasının engellenmesinin düzenlenmesinde rol oynarlar. Bu biyolojik aksiyonlarını membran guanil siklaz reseptörleri yoluyla düzenlemektedirler. Bu derlemede, natriüretik peptitlerin yapısı, fonksiyonları ve çeşitli sistemler üzerindeki etkileri anlatılmıştır.
Ozone treatment for high-dose systemic Steroid-Induced retinal injury
(Taylor & Francis Ltd, 2020) Yıldız, Aydın; Şehitoğlu, Müşerref Hilal; Karaboğa, İhsan; Arıkan, Sedat
Objective To investigate the effect of high-dose systemic steroids on retinal tissues and the effectiveness of ozone (O-3) therapy. Methods Twenty-four New Zealand white rabbits were divided into three groups of eight. Group 1 was accepted as the control group, Group 2 received intramuscular 20 mg/kg methylprednisolone acetate and Group 3 received 14 sessions of ozone treatment in addition to methylprednisolone acetate. The subjects were sacrificed on the 30(th)day. Retinal tissues were removed. Superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS) levels were evaluated for tissue biochemistry and serum ischaemic modified albumin (IMA), interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) levels were evaluated with the ELISA method. Haematoxylin-eosin staining and TUNEL evaluation for apoptosis were evaluated as histopathological methods. Results In the treatment group, antioxidant parameters of TAS, SOD and CAT were higher, oxidative and ischaemic parameters of MDA, TOS and IMA were lower, inflammatory parameters of IL-6 and TNF-alpha were lower, retinal thickness was better and apoptosis amount was lower. Conclusion Apoptosis increases in retinal tissues due to high dose systemic steroid administration and the retina becomes thinner. With biochemical examination, oxidation parameters increased while antioxidant parameters decreased. Both histopathological and biochemical parameters improved significantly with ozone treatment.
Protective effect of gel form of gastric gavage applicated aloe vera on ischemia reperfusion injury in renal and lung tissue
(C M B Assoc, 2017) Şahin, Hasan; Yener, Ali Ümit; Karaboğa, İhsan; Şehitoğlu, Müşerref Hilal; Doğu, Tuğba; Altınışık, Hatice Betül; Şimşek, Tuncer
The aloe vera plant has become increasingly popular in recent years. This study aimed to research the effect of aloe vera to prevent renal and lung tissue damage in an experimental ischemia-reperfusion (I/R) injury model. The study included 21 male Wistar Albino rats, which were categorized into control group, n = 7 (no procedures), Sham group n = 7 (I/R); and aloe vera therapy group, n = 7 (aloe vera and I/R). Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and malondialdehyde (MDA) were evaluated from lung and kidney tissues for biochemical investigations. As histopathological, hematoxylin and eosin and anti-iNOS were also examined. In biochemical investigations, SOD, CAT, and GPx levels of the Sham group were found to be lower compared with the other groups (P < 0.05). The aloe vera therapy group was not statistically different from control groups but significantly different compared with the Sham group. In the same way, the MDA levels of kidney and lung tissues were statistically significant in the aloe vera therapy group, compared to the Sham group. In the Sham group, the peribronchial and perialveolar edema were observed in lung parenchyma. Also, excess interstitial hemorrhage, leukocyte infiltration, and alveolar wall thickening were identified in ischemic groups. The histopathological changes were much lighter than in the aloe vera therapy group. In renal tissues, excess epithelial cell deterioration, tubular desqumination, and glomerular atrophy were observed in the Sham group. The histopathological changes were markedly reduced in the aloe vera therapy group. In the kidney and lung tissue, the level of iNOS activity in the Sham group was significantly higher than in the control and aloe vera therapy group. This study indicated that aloe vera is protective against oxidative damage formed by I/R in distant organs like the lungs and kidneys.
Protective Effects of Hesperetin Against Lipopolysaccharide-induced Acute Renal Injury in Rat
(Galenos Publ House, 2022) Kaya, Serkan; Karaboğa, İhsan
Objective: In this study, it was aimed to investigate the effects of hesperetin on renal transforming growth factor-beta 1 (TGF-beta 1) expression and apoptosis in rat lipopolysaccharide (LPS)-induced acute renal injury model. Methods: In the study, 18 adult male Wistar albino rats were used. Rats divided into three groups, respectively (n=6); control, LPS and LPS + hesperetin. Sepsis model was created with a singledose of LPS (Escherichia coli, O26: B6 serotype, Sigma-aldrich). LPS + hesperetin group was administered intragastrically with the aid of hesperetin oral gavage at a dose of 100 mg/kg, after LPS-induction. Twenty four hours after LPS administration, the rats were opened from the midline under ketamine-xylazine anesthesia and kidney tissue and cardiac blood were collected. Kidney tissue was examined with hematoxylin-eosin staining. TGF-beta 1 expression was determined by indirect immunohistochemical method. TUNEL method was used to determine renal apoptosis. Blood urea nitrogen (BUN) and creatinine levels in blood serum were determined using spectrophotometric methods. Results: Decreased histopathological changes, TGF-beta 1 expression and apoptosis were determined in the LPS + hesperetin group compared to the LPS group (p<0.05). In addition, a significant decrease in BUN and creatinine levels was observed in the LPS + hesperetin group compared to the LPS group (p<0.05). Conclusion: The data obtained show that in the LPS-induced rat sepsis model, hesperetin suppresses the expression of TGF-beta 1 in kidney tissue and provides a protective effect.
Protective effects of hesperetin on lipopolysaccharide-induced acute lung injury in a rat model
(Baycinar Medical Publishing, 2020) Kaya, S.; Kaya, S.A.; Polat, E.; Erboğa, Zeynep Fidanol; Duran, Y.; Polat, Fatin Rüştü; Karaboğa, İhsan
Background: In this experimental study, we aimed to investigate the effects of hesperetin, a natural flavonoid, on a lipopolysaccharideinduced acute lung injury model in rats. Methods: Between March 2019 and May 2019, a total of 18 adult male Wistar albino rats, weighing approximately 250 to 300 g, were randomly divided into three groups as control, lipopolysaccharide, and lipopolysaccharide + hesperetin groups (n=6 in each group). The wet/dry weight ratio of lung tissue was determined. Histopathological changes were examined using light and scanning electron microscopy. Pulmonary nuclear factor-kappa beta, inducible nitric oxide synthase, and alpha-smooth muscle antigen activity were determined with indirect immunohistochemical methods. Pulmonary apoptosis was detected with the terminal deoxynucleotidyl transferase dUTP nick-end labeling method. Tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and interleukin-10 concentrations were measured with enzyme-linked immunosorbent assay. Results: Treatment with hesperetin significantly improved the architecture of lung tissue and reduced the wet/dry weight ratio, nuclear factor-kappa beta, inducible nitric oxide synthase, and alphasmooth muscle antigen expression, pulmonary apoptosis, and levels of proinflammatory cytokines. Conclusion: Our study results suggest that hesperetin has a potent protective effect against lipopolysaccharide-induced acute lung injury in rats via suppression of the proinflammatory cytokine cascade, nuclear factor-kappa beta, signaling pathway activation, and apoptosis. © 2020 All right reserved by the Turkish Society of Cardiovascular Surgery.
Royal jelly attenuates gastric mucosal injury in a rat ethanol-induced gastric injury model
(Springer Science and Business Media B.V., 2020) Duran, Y.; Karaboğa, İhsan; Polat, Fatin Rüştü; Polat, E.; Erboğa, Zeynep Fidanol; Ovalı, M. A.; Yılmaz, A.; Çelikkol, A.
The aim of the study was to investigate traditionally used Royal Jelly (RJ) for treating an ethanol-induced gastric ulcer model in rats. A total of 32 Wistar albino male rats were divided into 4 groups of 8: group I = Control, group II = Ethanol, group III = RJ + Ethanol, and group IV = Lansoprazole + Ethanol. In groups II, III, and IV, animals were administered 1 ml of absolute ethanol orally after a 24-h fast to induce ulcer formation. The histopathological changes in the gastric mucosa were determined using hematoxylin-eosin (H&E) staining. Immunohistochemically, inducible nitric oxide (iNOS) and nuclear factor kappa beta (Nf-??) markings were evaluated in gastric tissue. Cell death in the gastric mucosa was determined by the TUNEL method. Oxidative status markers, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and myeloperoxidase (MPO) levels were determined spectrophotometrically. Expression of the interleukin – 1 beta (IL-1?) and tumor necrosis factor-? (TNF-?) genes in gastric tissues was determined by real-time PCR; and TNF-?, IL-10, and IL-1? levels were determined. RJ was found to inhibit iNOS and Nf-?? activity in the gastric mucosa and prevent epithelial cell apoptosis. In particular, pro-inflammatory cytokines TNF-? and IL-1? levels were significantly decreased in the RJ + Ethanol group compared to the Ethanol group. In addition, a decrease in the MPO level indicated that RJ prevented tissue damage, especially by preventing inflammatory cell infiltration. The study demonstrated a possible gastroprotective effect of RJ in a rat ethanol-induced gastric ulcer model. © 2020, Springer Nature B.V.

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