Kafeik Asit Fenetil Ester Sıçanlarda Göğüs Travması Sonrası Gelişen Hepatik Hasarı Nükleer Faktör Kappa Beta ve İndüklenebilir Nitrik Oksit Sentezini Baskılayarak Azaltır
Yükleniyor...
Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışmada göğüs travması modeli oluşturulansıçanlarda meydana gelen hepatik hasara karşı kafeik asitfenetil ester (KAFE)' nin Nükleer Faktör Kappa Beta (Nf-??)ve indüklenebilir nitrik oksit sentaz (iNOS) aktivitesi üzerineetkisinin incelenmesi amaçlandı.Yöntem: Çalışmada 40 adet erişkin Wistar albino erkek sıçansırasıyla 4 gruba ayrıldı; kontrol, göğüs travma modeli, göğüstravma modeli+KAFE ve KAFE grubu. KAFE uygulaması 7gün boyunca 10 µmol/kg/gün dozda intraperitoneal olarakgerçekleştirildi. Deney süresi sonunda karaciğer dokusualınarak meydana gelen histopatolojik değişikliklerHematoksilen-Eozin (H&E) boyaması ile değerlendirildi.Ayrıca hepatik Nf-?? ve iNOS aktiviteleri indirekimmünohistokimyasal yöntemle incelendi.Bulgular: Göğüs travması uygulanan grupta hepatositkordonlarında dejenerasyonları ve sinüzoidal dilatasyonbulgularına rastlandı. Göğüs travması+KAFE grubundahistopatolojik bulguların göğüs travması grubuna göre dahahafif seyrettiği belirlendi. Ayrıca, göğüs travması+KAFEgrubunda, göğüs travması grubuna kıyasla hepatik Nf-?? veiNOS immünreaktivitesinde istatistiksel olarak anlamlıderecede bir azalma olduğu görüldü (p<0.05).Sonuç: KAFE' nin göğüs travması uyarımlı sıçan hepatikhasarında Nf-?? ve iNOS aktivitesini baskılayarak koruyucuetki gösterdiği belirlendi.
Objective: The aim of this study was to investigate the effect of caffeic acid phenethyl ester (CAPE) on Nuclear Factor Kappa Beta (Nf-??) and inducible nitric oxide synthase (iNOS) activity against hepatic injury with chest trauma model in rats. Method: In the study, 40 adult Wistar albino male rats were divided into 4 groups respectively; control, chest trauma, chest trauma+CAPE and CAPE groups. CAPE administration was performed intraperitoneally at a dose of 10 ?mol / kg / day for 7 days. At the end of the experiment, histopathologic changes were determined by hematoxylin-eosin (H&E) stain in liver tissue. In addition, hepatic Nf-?? and iNOS activities were examined by indirect immunohistochemical method. Results: Degeneration of hepatocyte cords and sinusoidal dilatation findings were found in the chest trauma group. Histopathological findings of chest trauma + CAPE group were found to be milder than chest trauma group. In addition, a statistically significant reduction in hepatic Nf-?? and iNOS immunoreactivity was seen in the chest trauma + CAPE group compared to the chest trauma group (p<0.05). Conclusion: CAPE has been shown to exert protective effects by suppressing Nf-?? and iNOS activity in chest trauma induced hepatic damage in rats.
Objective: The aim of this study was to investigate the effect of caffeic acid phenethyl ester (CAPE) on Nuclear Factor Kappa Beta (Nf-??) and inducible nitric oxide synthase (iNOS) activity against hepatic injury with chest trauma model in rats. Method: In the study, 40 adult Wistar albino male rats were divided into 4 groups respectively; control, chest trauma, chest trauma+CAPE and CAPE groups. CAPE administration was performed intraperitoneally at a dose of 10 ?mol / kg / day for 7 days. At the end of the experiment, histopathologic changes were determined by hematoxylin-eosin (H&E) stain in liver tissue. In addition, hepatic Nf-?? and iNOS activities were examined by indirect immunohistochemical method. Results: Degeneration of hepatocyte cords and sinusoidal dilatation findings were found in the chest trauma group. Histopathological findings of chest trauma + CAPE group were found to be milder than chest trauma group. In addition, a statistically significant reduction in hepatic Nf-?? and iNOS immunoreactivity was seen in the chest trauma + CAPE group compared to the chest trauma group (p<0.05). Conclusion: CAPE has been shown to exert protective effects by suppressing Nf-?? and iNOS activity in chest trauma induced hepatic damage in rats.
Açıklama
Anahtar Kelimeler
Kaynak
Van Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
26
Sayı
2
