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Öğe Horse Surgery and Survival Prediction with Artificial Intelligence Models: Performance Comparison of Original, Imputed, Balanced, and Feature-Selected Datasets(Kafkas Univ, Veteriner Fakultesi Dergisi, 2024) Cihan, PinarArtificial intelligence (AI) technology, while less advanced than in human medicine, holds significant potential in the field of veterinary medicine. This technology offers a range of essential benefits, such as disease diagnosis, treatment planning, disease control, and overall animal health improvement. Based on clinical data, this study uses 15 AI models to predict the necessity of surgery and the likelihood of survival in horses displaying symptoms of acute abdominal pain (colic). By comparing surgical and survival predictions across the original, imputed missing values, and balanced datasets, we determine the most effective dataset based on the average accuracy of the 15 AI models. Furthermore, we explore the potential for improved accuracy with a reduced feature set by calculating feature importance scores for surgery and survival predictions. Our results indicate that the balanced dataset achieved the highest average accuracy for predicting surgery and survival, with 80.76% and 77.96%, respectively. The Random Forest (RF) model outperformed others as the most accurate model for both surgery (accuracy = 85.83, Area Under the Curve [AUC] = 0.906) and survival prediction (accuracy = 80.75, AUC = 0.888). It was observed that reducing the number of features in the dataset by 56% led to an increase in surgery prediction accuracy to 86.38%. Similarly, when the number of features was reduced by 24% for survival prediction, the prediction performance increased to 83.75%. This study emphasizes the importance of the precise implementation of artificial intelligence techniques in veterinary medicine, which can significantly enhance model performance.Öğe The Change of Oral Presentations of National Anatomy Congresses Over the Years(Pera Yayincilik Hizmetleri, 2023) Ozkan, Mazhar; Dincel, Yasar MahsutIntroduction: In this study, it is aimed to examine the characteristics and publication rates of oral presentations presented in national congresses. Methods: The data about the oral presentations in national anatomy congresses (from 2016 to 2021), including the number of contributing institutions, the institutions that contributed the most oral presentations each year, and the publication rates of the oral presentations in the journals scanned in different indexes. In the study, the titles and the keywords were scanned using Google Scholar and Web of Science, and journal index details were noted. Results: Of the national congresses we examined in our study, the most oral presentations (193) were the congress in 2019 and the least oral presentations (47) were the congress in 2017. The publication rate of oral presentations was highest in the papers of the congress in 2016 (42%) and at least in the papers of the congress in 2019 (13%). None of the oral presentations in the last congress held in 2021 were published until the time of our study. Large proportions (34%) of the papers that are accepted as oral presentations and published are radiological studies. 70% of the publications were published in journals indexed in Web of Science. Conclusion: For both new and senior academics, congresses offer a useful and distinctive setting. Congress papers showcase the scientific opportunities and interests of various universities while also providing opportunities for collaboration. Attendance at a conference is significantly influenced by its location, timing, and financial situation.Öğe Investigation of the Effect of Tinnitus and Hearing Loss on Hippocampus Volume(Pera Yayincilik Hizmetleri, 2024) Sasani, Hadi; Ozkan, Mazhar; Ersozlu, TolgaObjective: This study aims to compare hippocampal changes with a correlation of audiological testing results in patients suffering from tinnitus. Methods: Patients diagnosed with tinnitus in the university hospital between February 2021 and March 2022 were prospectively included in the study by performing magnetic resonance imaging. The volume was determined by manually tracing the hippocampus' margins on the images using the Vitrea2 (R) workstation (Canon Medical Systems Vital Images, Minnesota, USA). Statistics were used to assess the correlation between the parameters of the hearing test. Results: The distribution of the patient group (21 males, 19 females) and control group (15 males, 15 females) was uniform, and the mean ages of the two groups were 50.23 +/- 12.09 and 32.30 +/- 7.97, respectively. Significant statistical differences existed in the mean ages of the groups (p<0.05). Bilateral hippocampal volumes, right bone, and air conduction all differed significantly (p<0.05). The median values in the patient group were as follows: right HC 2620 mm3 (range 1600-3610), left HC 2450 mm3 (range 1610-3990), right air conduction 20 dB (range 10-61), left air 21 dB (range 11-65), and right bone 13.5 dB (range 8-49). Age was positively correlated with bilateral measurements of air and bone hearing levels (p 0.05; right air r=0.513, right bone r=0.438, left air r=0.589, left bone r=0.487). Between the 30-39 and 60-69 age groups, there was a significant difference in bone and air conduction levels (p<0.05). Conclusion: In this study, it was found that the hippocampus volumes of healthy hearing people with tinnitus complaints were significantly higher in MRI examinations compared to the control group. In addition, in cases of tinnitus accompanied by bone conduction hearing loss, hippocampus volumes were found to be less than those of tinnitus alone, but not less than in the control group. It is suggested that chronic acoustic stimulation caused by tinnitus causes an increase in hippocampus volume and that problems in sensorineural integrity prevent this increase.Öğe Discussion on the Artificial Intelligence (AI) Tools Usage in the Scientific World(Pera Yayincilik Hizmetleri, 2023) Ozkan, Mazhar; Sasani, Hadi[Abstract Not Available]Öğe Pain types and risk factors in post-COVID-19(Baycinar Medical Publ-Baycinar Tibbi Yayincilik, 2024) Ketenci, Aysegul; Zure, Mert; Akpinar, Fatma Merih; Ozdemir, Yelda Soluk; Balbaloglu, Oezlem; Akaltun, Mazlum Serdar; Erden, EnderObjectives: This study aims to accurately evaluate pain lasting longer than three months and falls under the category of chronic pain and to determine the risk factors to follow up and treat properly and to develop appropriate diagnostic and treatment algorithms. Patients and methods: Between March 2021 and December 2021, a total of 437 patients (162 males, 275 females; mean age: 44 +/- 14.6 years; range, 12 to 82 years) who were referred to the participating centers due to pain complaints and were diagnosed with post-COVID-19 condition according to the criteria defined by the World Health Organization (WHO) were included in the study. The patients were divided into three groups as nociceptive pain, neuropathic pain, and central sensitization, based on the physician's clinical evaluation and the Self-Report Leeds Assessment of Neuropathic Symptoms and Signs (S-LANSS) and Central Sensitization Inventory scores. Results: The most common diagnosis was nociceptive pain followed by central sensitization. Patients with nociceptive pain had less pain. It was found that not exercising regularly, having a chronic disease and being a woman were risk factors for central sensitization, having thyroid disease before COVID-19, and defining the current pain as very severe were risk factors for neuropathic pain. Conclusion: In the evaluation of post-COVID-19 pain, neuropathic pain and central sensitization should be also considered in addition to nociceptive pain and the severity of pain, systemic diseases and physical activity should be questioned.Öğe An unusual clinical presentation of nontraumatic myositis ossificans: Leg length discrepancy(Baycinar Medical Publ-Baycinar Tibbi Yayincilik, 2024) Sirin, Akif; Sarifakioglu, Banu; Ustaoemer, Kuebra; Yilmaz, ErtanFunctional leg length discrepancy results from soft tissue tightness or weakness across any joint in the lower extremity or spine. Herein, we present a 23-year-old female patient with leg length discrepancy due to a nontraumatic myositis ossificans (MO). Interpretation of the imaging findings is quite decisive in diagnosing soft tissue pathologies. It is particularly valid for MO to differentiate from other malignant or infectious lesions. There is no consensus on the treatment of nontraumatic MO. Although there are studies stating the contrary, surgical interventions should be considered as second option for patients who failed with nonsurgical treatments such as physical therapy.Öğe Fluid-based wearable sensors: a turning point in personalized healthcare(Tubitak Scientific & Technological Research Council Turkey, 2023) Vural, Berin; Uludag, Inci; Ince, Bahar; Ozyurt, Canan; Ozturk, Funda; Sezginturk, Mustafa KemalNowadays, it has become very popular to develop wearable devices that can monitor biomarkers to analyze the health status of the human body more comprehensively and accurately. Wearable sensors, specially designed for home care services, show great promise with their ease of use, especially during pandemic periods. Scientists have conducted many innovative studies on new wearable sensors that can noninvasively and simultaneously monitor biochemical indicators in body fluids for disease prediction, diagnosis, and management. Using noninvasive electrochemical sensors, biomarkers can be detected in tears, saliva, perspiration, and skin interstitial fluid (ISF). In this review, biofluids used for noninvasive wearable sensor detection under four main headings, saliva, sweat, tears, and ISF-based wearable sensors, were examined in detail. This report analyzes nearly 50 recent articles from 2017 to 2023. Based on current research, this review also discusses the evolution of wearable sensors, potential implementation challenges, and future prospects.Öğe Investigation of the Effect of Farnesol on Biofilm Formation by Candida albicans and Candida parapsilosis Complex Isolates(Ankara Microbiology Soc, 2024) Erdal, Berna; Baylan, Bensu; Batar, Bahadir; Ozturk, Ali; Topcu, BirolThe incidence of infections caused by Candida species has significantly increased over the past three decades. Candida albicans is commonly recognized as the primary causative agent in cases of candidiasis; however, non-albicans Candida species, including Candida parapsilosis, are also frequently defined as pathogens. Treatment -resistant infections arise as a result of biofilm formation, which is one of the effective mechanisms in the pathogenesis of Candida infections. However, the mechanisms of action of farnesol, a quorum sensing (QS) system molecule, on biofilm formation by Candida species remain unclear. This study aimed to demonstrate the changes in the biofilm biomass of C.albicans and C.parapsilosis complex isolates in the presence of farnesol and reveal the expression of the EFG1 and BCR1 genes, which are believed to play a role in the production of QS molecules, using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis. C.albicans (n= 91) and C.parapsilosis complex (n= 29) isolates obtained from different clinical samples were included in the study. The minimum inhibitory concentration (MIC) values of farnesol were determined using the broth microdilution method according to the M27 -A3 protocol of the Clinical and Laboratory Standards Institute (CLSI). The biofilm biomass of the isolates was examined without farnesol and at the MIC-0 and MIC-2 concentrations of farnesol. Changes in the expression of the biofilm-associated EFG1 and BCR1 genes were investigated using qRT-PCR. According to the results of the study, the MIC values of farnesol were detected in the range of 1-2 mM in 82.4% (n= 75) of the C.albicans isolates and in the range of 0.5-1 mM in 72.4% (n= 21) of the C.parapsilosis complex isolates. Of the C.albicans isolates, 27 (29.7%) exhibited a strong biofilm formation and 58 (63.7%) demonstrated a weaker biofilm formation, while these rates were 34.4% (n= 10) and 62.1% (n= 18), respectively, for the C.parapsilosis complex isolates. At the MIC-0 and MIC-2 concentrations, farnesol was observed to reduce biofilm biomass among C.albicans (n= 24, 88.9%) and C.parapsilosis complex (n= 8, 80.0%) isolates that formed strong biofilms and observed to increase biofilm biomass among those that formed weak biofilms [60.3% (n= 35) and 55.6% (n= 10), respectively]. On completion of the qRT-PCR analysis supporting the results of the biofilm experiment, it was determined that the expressions of the EFG1 and BCR1 genes decreased at the MIC-0 and MIC-2 concentrations of farnesol among the strong biofilm-forming C.albi- cans and C.parapsilosis complex isolates, but there was an increase in gene expressions among the weak biofilm-forming isolates. In addition to the antifungal effect of farnesol on Candida species, this study provided data on the efficacy of the MIC-0 and MIC-2 concentrations of farnesol against Candida biofilm biomass. Although our results suggest that farnesol can be used as an alternative agent to reduce biofilm formation in Candida infections, they need to be supported by further studies. Moreover, this research has significance as it represents the first study to determine the EFG1 and BCR1 gene expressions among C.parapsilosis complex isolates in the presence of farnesol.Öğe Brown rust resistance screening and molecular characterization of wheat cultivars by molecular markers(Tubitak Scientific & Technological Research Council Turkey, 2023) Bilgen, Behiye Banu; Yuruk, Busra; Nasirian, HazalTriticum aestivum L. is one of the major strategic crops for many reasons such as being the basic material for the majority of nutrients, being a source of energy, a source of protein, and also other organic and inorganic compounds for both humans and animals. The genetic characterization is useful for designing plant breeding programs for many plants, including wheat. The selection of genotypes resistant to stress (biotic and abiotic) conditions is another important point for the design of plant breeding programs. Wheat brown (leaf) rust is one of the major significant biotic stress factors. In this study, marker analysis for brown rust resistance for Lr13, Lr14a, Lr19, Lr22a, Lr24, Lr34, Lr37, and Lr47 genes was performed, and the genetic characterization of 96 wheat genotypes was determined by 8 SSR and 4 ISSR loci. Eighteen alleles were determined for SSR loci (polymorphism ratio of 37.5%) and a total of 33 polymorphic bands (polymorphism ratio of 82.5%) were determined for ISSR loci. The mean Shannon Index was estimated as I (SSR) = 0.298 and I (ISSR) = 0.346. The mean gene diversity for SSR and ISSR was estimated as 0.407 and 0.246, respectively. The genetic similarity value based unweighted pair group method with arithmetic was used to form the dendrogram and it was remarked wheat varieties were grouped into two main clusters. The STRUCTURE analysis also separated the studied wheat genotypes into two groups. The results reveal the genetic structure, the genetic diversity, and the presence of resistance alleles of used wheat varieties, and the selection of genetically diverse and leaf rust-resistant genotypes can serve as valuable parents for future wheat breeding or improving breeding programs.Öğe Determination of some environmental factors on milk yield traits and milk components in Simmental cows(Tubitak Scientific & Technological Research Council Turkey, 2024) Sahin, Onur; Soysal, Mehmet Ihsan; Gurcan, Eser KemalThe aim of the study was to determine the effects of various environmental factors on milk yield and milk components in Simmental cows. In the present study, 33,813 milk yield records from 1631 cows and milk samples from 233 cows were used for milk component analysis. Lactation milk yield (LMY) and 305 -day yield (305-DMY) were the milk yield characteristics studied. Environmental factors considered for milk yield characteristics included province, calving year, calving month, parity, age, and lactation length. Milk components analyzed were fat percentage (FP), protein percentage (PP), lactose percentage (LP), somatic cell count (Log(10)SCC), and dry matter (DM). Environmental factors considered for milk components were province, birth year, control month, calving month, and parity. The results indicated that all environmental factors significantly influenced milk yield characteristics (p < 0.01). Specifically, province and birth year significantly affected FP and Log(10)SCC (p < 0.01), whereas calving month, control month, and parity did not significantly affect FP. Province, birth year, and control month significantly influenced PP and DM (p < 0.01), while calving month and parity did not significantly affect PP. Province was the only significant factor for LP (p < 0.01). Considering all data, the averages of traits were as follows: LMY (5628.1 +/- 12.80 kg), 305-DMY (5309.2 +/- 11.80 kg), total dry matter yield (TDMY) (22.58 +/- 0.51 kg), DM (12.43 +/- 0.08%), Log10SCC (2.29 +/- 0.04, equivalent to 226239 cells/mL), FP (3.78 +/- 0.06%), PP (3.57 +/- 0.02%), and LP (4.72 +/- 0.01%).Öğe Using computational modeling to design antiviral strategies and understand plant-virus interactions(Tubitak Scientific & Technological Research Council Turkey, 2024) Kamal, Hira; Zafar, Muhammad Mubashar; Razzaq, Abdul; Ijaz, Aqsa; Anwar, Zunaira; Topcu, Hayat; Elhindi, Khalid M.Using a bioinformatics approach to identify binding pockets between proteins is a preferable method before modifying the genome to delineate host interactions with viruses. Based on extensive proteomics data in numerous databases, several interaction prediction methods are available to identify binding sites between viruses and hosts at the individual residue level, but little is known about the interaction prediction strategy for plant viruses. Begomoviruses, belonging to the family Geminiviridae, constitute a group of circular single-stranded (ss) DNA viruses that encode multifunctional proteins responsible for viral replication, causing severe diseases in multiple host plants. These viruses usually escape through plant defense mechanism overcoming physical and chemical barriers to trigger the infection with all possible combinations of interaction in the target host protein partners. Here, we have applied our computational approach for plant virus interaction at domain level. Previous study showed that myristoylation-like motif in Begomovirus cotton leaf curl Multan associated betasatellite protein beta C1 (CLCuMB- beta C1) played an important role for interaction with ubiquitin conjugating enzyme protein (UBC3) in tomato. This kind of binding at residue level has been validated using in-vivo and in-vitro molecular approach. Here, an in-silico approach was utilized which is a combinatorial source of previous and recent protein prediction methods to determine all possible identified interface sites between beta C1 and UBC3. This molecular interaction of CLCuMB-beta C1 was further verified in the actual host i.e. cotton using bimolecular fluorescence complementation system and yeast two hybrid assay. This combinatorial approach of computational and molecular data will help to identify the interaction between virus and host before using any expensive and time consuming molecular techniques.Öğe Dual-reactive hydrogels functionalizable using Huisgen click and Schiff base reactions(Tubitak Scientific & Technological Research Council Turkey, 2023) Cengiz, NergizHydrogels incorporating different reactive groups are important platforms for the fabrication of functional materials through the conjugation of diverse molecules. In this study, a dual-reactive hydrogel system was designed utilizing aldehyde and azide groups containing methacrylate monomers. Hydrogels were obtained in the presence of a dimethacrylate crosslinker with a combination of hydrophilic PEG-based monomers via free-radical polymerization. The azide and aldehyde sites of the hydrogel network are reactive towards alkyne and amine functional groups, respectively. The advantage of the different reactivities of these functional groups was demonstrated through the attachment of two different dye molecules onto the hydrogel platform via the Huisgen click and Schiff base reactions to obtain a sensing platform for various applications, such as indicating change in pH of the environment.Öğe Analyzes of algebraic classification of higher dimensional Kundt geometries with large D method(Tubitak Scientific & Technological Research Council Turkey, 2023) Kirezli, PinarIn this paper, the classification of higher dimensional Kundt geometry is revisited as the dimension of the spacetime D -> infinity. In addition to previous studies, in order to Kundt geometry becomes algebraically special spacetime obligatory conditions are determined. Additionally, Type II, Type III, Type N, Type O, and Type D Kundt geometries are explicitly analyzed. Classification of several metrics such as pp-waves, nongyratonic Kundt metric, and VSI spacetime, which are well-known subclasses of Kundt geometry are studied.Öğe Nuclear DNA content and ploidy levels of living apple germplasm collection in Türkiye(Tubitak Scientific & Technological Research Council Turkey, 2023) Kacal, Emel; Simsek, Yaren Ipek; Seymen, Turgay; Oezongun, Serif; Tuna, MetinOne of the key uses of flow cytometry is the estimation of nuclear DNA content and ploidy level, which has proven a reliable and effective approach in many studies. The primary goal of this research is to estimate the nuclear DNA content and ploidy level of the apple genetic resource collection maintained in the Fruit Research Institute's living Malus collection for the first time using flow cytometry (for autochthonous varieties). Fresh apple leaf tissues were used for the flow cytometry analysis. For each genotype, nuclear DNA analysis was performed on three individual plants. Propidium iodide (PI) is used as a fluorochrome. Common vetch (3.65 pg/2C) was used as an internal standard. The 2C nuclear DNA content ranged from 1.46 pg to 2.45 pg. The variation in nuclear DNA content within the collection was statistically significant. Apple genotypes were split into two groups, diploid and triploid, according to their nuclear DNA content. Based on these results, 16.47% of apple genotypes were triploid, while 83.53% were diploid. The 2C nuclear DNA content in triploid genotypes varied from 2.04 to 2.45 pg and in diploids from 1.46 to 1.69 pg. The average nuclear DNA content in diploids was 1.56 pg, whereas it was 2.29 pg in triploids. The results of the study will be useful to determine the best strategies in breeding programs, as ploidy is one of the most important characteristics to consider in selecting parents for breeding purposes in addition to their characteristics.Öğe The effect of papaverine on tendon healing and adhesion in rats following Achilles tendon repair(Turkish Joint Diseases Foundation, 2024) Can, Erdem; Dincel, Yasar Mahsut; Karabulut, Derya; Karabag, Sevil; Arslan, Yunus ZiyaObjectives: The study aimed to examine the histopathological and biomechanical effects of papaverine administered intraperitoneally and locally on Achilles tendon healing in a rat model. Materials and methods: Forty-eight adult male SpragueDawley rats (range, 300 to 400 g) were used in this study conducted between October and November 2022. The rats were divided into three groups, with each group further subdivided into two for sacrifice on either the 15(th) (early period) or 30(th) (late period) day after surgery. The first (control) group received no treatment following Achilles tendon repair, while papaverine was intraperitoneally administered every other day for 10 days in the second group and locally in the third group after surgery. On the 15(th) and 30(th) days, the rats were sacrificed, and their Achilles tendons were subjected to biomechanical testing and histopathological evaluation. Results: Histopathologically, there were no significant differences among the groups on the 15 th day. However, on the 30 th day, the locally applied papaverine group exhibited superior histopathological outcomes compared to the control group (p<0.05). Concerning the highest tensile strength values before rupture, the biomechanical assessment showed that the group receiving local papaverine treatment in the early period and both the group with systemic papaverine treatment and the one with local papaverine treatment in the late period displayed a statistically significant advantage compared to the control group (p<0.05). Conclusion: Locally administered papaverine has positive biomechanical effects in the early period and exhibits a positive correlation both histopathologically and biomechanically in the late period. Novel therapeutic options may be provided for patients through these findings.Öğe Comparison of the prophylactic use of ibandronate and its use in early-stage osteonecrosis in rats with steroid-induced osteonecrosis of the femoral head(Turkish Joint Diseases Foundation, 2023) Caglar, Sever; Dasnci, Mustafa Fatih; Acar, Abdurrahman; Caglar, Aysel; Dincel, Yasar Mahsut; Cataltepe, AzizObjectives: The aim of this study was to investigate the effects of ibandronate before and after the onset of osteonecrosis in rats with steroid-induced osteonecrosis of the femoral head.Materials and methods: A total of 24 female Sprague-Dawley rats were used in this study. Three groups were formed with eight rats in each group. The first group was the prophylactic group that received ibandronate treatment before and after the onset of osteonecrosis (Group PT). The second group received ibandronate treatment three weeks after the development of osteonecrosis (Group TAO). The third group was the control group in which osteonecrosis was created, but only normal saline (NS) was given. At the end of the study, all rats were sacrificed, and their right femoral heads were removed, fixed with formalin, and sent for micro-computed tomography. Hematoxylin-eosin (H & E) and immunohistochemical examinations of the right femoral head sections were performed.Results: In the PT group, the trabecular thickness was significantly higher compared to those of the TAO and control groups (p<0.05). The trabecular thickness did not significantly differ between the TAO and control groups. The trabecular bone pattern factor was significantly higher in the PT group compared to the control and TAO groups (p<0.05); however, it showed no significant difference between the TAO and control groups. The incidence of type 2 osteonecrosis in the PT and TAO groups was significantly lower than that in the control group (p<0.05). The incidence of tissue-non-specific alkaline phosphatase (TNAPase) immunoreactivity of osteoblast positivity was significantly higher in the PT and TAO groups compared to the control group (p<0.05), whereas the incidence of TRAPase immunoreactivity of osteoclastic positivity was significantly lower in the PT and TAO groups compared to the control group (p<0.05).Conclusion: Intravenous administration of ibandronate before the onset of the disease was more effective in the treatment of osteonecrosis in rats with steroid-induced osteonecrosis of the femoral head.Öğe Serum Soluble Urokinase Plasminogen Activator Receptor Levels in Resistant Hypertension(Aves, 2024) Bayrakci, Nergiz; Ozkan, Gulsum; Kara, Sonat Pinar; Yilmaz, Ahsen; Celikkol, AliyeBackground: Although the precise pathogenetic mechanisms remain incompletely elucidated, hypertension is characterized by endothelial damage and inflammation. The soluble urokinase plasminogen activator receptor (suPAR) is an inflammatory biomarker that increases in parallel with disease activity in conditions such as systemic inflammation, infection, cancer, and atherosclerosis. The objective of this study was to assess inflammation by means of serum suPAR levels in hypertensive patients and those with resistant hypertension. Methods: Eighty-six adults, 29 newly diagnosed hypertensive patients (HT group) and 23 resistant hypertensive patients (RHT group) and 34 healthy controls, were included in this cross-sectional, observational study. Ambulatory blood pressure monitoring results were included in the analysis. Serum suPAR levels were measured using Enzyme -Linked ImmonuSorbent Assay (ELISA). Patients were divided into 2 groups as high suPAR and low suPAR. Results: The serum suPAR level was lower in the control group than that of the HT and RHT groups (P = .001). Systolic and diastolic blood pressure values were positively correlated with the serum suPAR level (r = 0.254, P = .018; r = 0.239, P = .027, respectively). Being in the high-suPAR group was found to increase the risk of RHT by 19.5 times. Other risk factors for RHT were found to be lower urinary sodium excretion and higher urinary albumin excretion [odds ratio (OR) = 0.98; OR = 1.09, respectively]. Conclusion: In our study, suPAR levels were found associated with RHT. To our best knowledge, this study is the first to evaluate the relationship between serum suPAR levels and RHT.Öğe The Role and Antagonistic Effects of miR-16-5p in the Regulation of ADP-Ribosylation Factor-Like Tumor Suppressor Gene 1 in Lung Cancer Cells(Ataturk Univ, 2023) Yuksel, Tugba Nurcan; Bozgeyik, Esra; Bozgeyik, IbrahimObjective: ADP-ribosylation factor-like tumor suppressor gene 1 is a member of the Ras superfamily of small guanosine triphosphatases that are known to be involved in multiple regulatory pathways in the multistage development of human cancers. Also, ADP-ribosylation factor-like tumor suppressor gene 1 expression levels have been reported to be dramatically lower in both cancer cell lines and tumor tissues compared to con-trols. Accordingly, defects in the regulation of the ADP-ribosylation factor-like tumor suppressor gene 1 gene seems have key tumor suppressive effects in the formation and development of human cancers including lung cancer. Moreover, microRNAs regulating the expression of ADP-ribosylation factor-like tumor suppressor gene 1 have not been described previously. Accordingly, the present study aimed to reveal the influence of miR-16-5p on the regulation of ADP-ribosylation factor-like tumor suppressor gene 1 gene. Materials and Methods: A549 lung adenocarcinoma cells were used. For the overexpression and silencing experiments of miR-16-5p synthetic microRNA mimics and inhibitors were used, respectively. Gene expres-sion analyses were achieved with the help of quantitative real-time polymerase chain reaction. Results: MiR-16-5p was identified to be predictive target of ADP-ribosylation factor-like tumor suppressor gene 1 and directly targets the expression of ADP-ribosylation factor-like tumor suppressor gene 1 as revealed by the overexpression and silencing experiments. Specifically, it was found that miR-1 6-5p- overe xpres sed A549 cells showed a decrease in ADP-ribosylation factor-like tumor suppressor gene 1 gene expression, whereas miR-16-5p-suppressed cells showed an increase in expression. These findings possibly suggest that miR-16-5p is the direct regulatory microRNA that posttranscriptionally regulates the expression of ADP-ribosylation factor-like tumor suppressor gene 1. Conclusion: Collectively, miR-16-5p seems to be a key regulatory molecule involved in the posttranscrip-tional regulation of the ADP-ribosylation factor-like tumor suppressor gene 1, and it might be responsible for the downregulation of this gene in lung cancer.Öğe Influence of Genotype and Housing Systems on the Incidence of White Striping, Proximate Composition, and Sensory Analysis of Broiler Breast Meat(Aves, 2023) Ozbek, Melahat; Petek, Metin; Cetin, Ece; Cetin, IsmailThis study was carried out to investigate the influence of genotype and housing systems on the incidence of white striping, proximate composition, and sensory analysis of broiler breast meat. In total, 10 random breast muscle samples of male broilers from each of 6 interactive groups (fast and slow-growing broilers x deep litter, fully slatted flooring and free-range housing; 2 x 3:6), slaughtered at 56 days old, were collected and analyzed in the study. The proximate composition analysis was evaluated using the AOAC Official Methods of Analysis. The occurrence of white striping was determined by examining the pectoral muscles of the broiler in the groups. A 9-point hedonic scale was used for sensory analysis of meat samples. The crude ash, crude fat content, and cooking loss values of the fast-growing broilers meat samples were found greater than slow-growing broilers' meat (p < .02, p < .001, and p < .033), while meat samples of slow-growing broiler had significantly higher crude protein and water-holding capacity values (p < .001 and p < .002). The crude fat content of free-range meat and crude protein of the meat produced from the slatted floors were significantly greater than the others (p < .001 and p < .043). The prevalence of white striping in breast meat in fast-growing broilers was significantly greater than that of slow-growing broilers. The meat produced from the slatted floor had significantly greater values for odor intensity, flavor intensity, and overall acceptability than the meat produced from free-range and deep litter housing (p < .012, p < .017, and p < .006). In conclusion, it can be said that the housing system, genotype, and genotype x housing system interactions affected the broiler's breast meat quality characteristics. All nutritional characteristics of broiler breast meat and the occurrence of white striping are significantly affected by genotype. Planning further research in commercial conditions should be more useful to see the comprehensive effects of the factors investigated in this experiment.Öğe Detection of Feline Coronavirus, Feline Immunodeficiency Virus, Feline Leukemia Virus, and Other Pathogen Genetic Material in Whole Blood From Domestic Cats in Turkiye(Aves, 2023) Muz, Dilek; Muz, Mustafa NecatiMany pathogens threaten feline health at all ages. Some pathogens suppress the immune system in cats, induce immunodeficiency, and predispose cats to other pathogens. In this study, 150 clinically sick (n = 119) and healthy-looking (n = 31) cats presented to private veterinary clinics with suspicious feline infectious peritonitis were sampled. Feline coronavirus, feline calicivirus, feline herpesvirus-1, feline immunodeficiency virus, feline leukemia virus, parvovirus, and Anaplasma species were investigated using pathogen-specific polymerase chain reaction protocols. Analysis results and demographic information of cats were evaluated. According to results, the positivity rates of feline coronavirus, feline leukemia virus, Anaplasma sp., feline immunodeficiency virus, parvovirus, feline calicivirus and feline herpesvirus were detected as 23.3%, 54%, 46%, 26%, 6.7%, 6% and 2.7%, respectively. The 11 cats (7.3%) were defined for wet feline infectious peritonitis. This research is the first report regarding feline herpesvirus-1, feline calicivirus, and parvovirus infections in cats in Tekirdag province. The results showed that viral agents and co-infections are common in domestic cats. Pathogen positivity was higher at younger ages and in clinically sick cats. The possibility of the coexistence of multiple pathogens in cats applying to the clinics should be considered for treatment success and the regulation of vaccination programs.
