Effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid-induced experimental colitis model
Yükleniyor...
Dosyalar
Tarih
2018
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Cellular and Molecular Biology Association
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In our study, the effect of hesperetin on inflammatory and oxidative status in trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis model was investigated through different methods. Eighteen Wistar albino male rats were divided in to three groups: Group I (Control, n = 8; 1 ml physiological saline), Group II (Colitis, n = 8; 1 ml TNBS), Group III (Hesperetin, n = 8; 1 ml TNBS and 100 mg/kg hesperetin). Macroscopic and microscopic scores were calculated to determine the damage to the colon at the end of the experiment. Serum tumor necrosis factor-? (TNF-?) and tissue interleukin-6 (IL-6) levels were determined using the ELISA method. Myeloperoxidase (MPO), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels were investigated spectrophotometrically. The TUNEL method was used for the detection of apoptotic cells in the colon tissue. Inducible nitric oxide synthase (iNOS) and nuclear factor-kappa-B (NF-??) expression in the colon were determined immunohistochemically. Hesperetin administration has shown to significantly reduce levels of MPO, MDA, and proinflammatory agents (TNF-?, IL-6, and NF-??). It has also been proven to inhibit mucosal apoptosis. This study indicates that hesperetin is protective against TNBS-induced colitis model via antiinflammatory, antioxidant and antiapoptotic effects. © 2018 by the C.M.B. Association. All rights reserved.
Açıklama
Anahtar Kelimeler
Hesperetin, IL-6, INOS, MPO, NF-??, TBNS-induced colitis, catalase, hesperetin, immunoglobulin enhancer binding protein, inducible nitric oxide synthase, interleukin 6, malonaldehyde, myeloperoxidase, superoxide dismutase, tumor necrosis factor, antioxidant, hesperetin, hesperidin, immunoglobulin enhancer binding protein, inducible nitric oxide synthase, interleukin 6, malonaldehyde, peroxidase, superoxide dismutase, trinitrobenzenesulfonic acid, tumor necrosis factor, animal experiment, animal model, animal tissue, antiinflammatory activity, antioxidant activity, apoptosis, Article, colon mucosa, colon ulcer, controlled study, enzyme linked immunosorbent assay, immunohistochemistry, lipid peroxidation, nonhuman, oxidative stress, rat, trinitrobenzene sulfonic acid-induced colitis, TUNEL assay, animal, chemically induced, colitis, drug effect, male, metabolism, Wistar rat, Animals, Antioxidants, Colitis, Enzyme-Linked Immunosorbent Assay, Hesperidin, Immunohistochemistry, In Situ Nick-End Labeling, Interleukin-6, Lipid Peroxidation, Male, Malondialdehyde, NF-kappa B, Nitric Oxide Synthase Type II, Oxidative Stress, Peroxidase, Rats, Rats, Wistar, Superoxide Dismutase, Trinitrobenzenesulfonic Acid, Tumor Necrosis Factor-alpha
Kaynak
Cellular and Molecular Biology
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
64
Sayı
11
