Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf-?? activation in blunt pulmonary contusion model

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Küçük Resim

Tarih

2019

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Turkish Assoc Trauma Emergency Surgery

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

BACKGROUND: Pulmonary contusion (PC) is an important life-threatening clinical condition characterized by lung injury and inflammation. Caffeic acid phenethyl ester (CAPE) is a biological agent with potent antioxidant and anti-inflammatory effects. This study aimed to investigate the potential effects of CAPE on tissue damage, nuclear factor kappa-beta (Nf-kappa beta) activity, inducible nitric oxide synthase (iNOS) synthesis, and pulmonary apoptosis in an experimental PC model. METHODS: Forty adult Wistar albino rats were used in this study and divided into four groups as follows: control, PC, PC + CAPE, and CAPE. CAPE was administered intraperitoneally for seven days following PC formation (10 jimol/kg, dissolved in dimethyl sulfoxide). Wet/dry weight ratio in lung tissue was determined. The pulmonary tissue was examined using hematoxylin-eosin and Masson's trichrome histochemical staining and also by scanning electron microscopy. Nf-kappa beta and iNOS activities in the lungs were determined by the indirect immunohistochemical method. Pulmonary apoptosis was detected by the TUNEL method. RESULTS: Increased leukocyte infiltration score, pulmonary edema, alveolar damage, and increased Nf-kappa beta and iNOS activities were determined in the PC group. CAPE administration inhibited Nf-kappa beta and iNOS activities and pulmonary apoptosis. CONCLUSION: In this study, the findings showed that CAPE inhibited tissue damage by suppressing inflammatory mediators of Nf-kappa beta and iNOS activities. Also, CAPE was found to be protective in the lung tissue and could be used as a therapeutic agent.

Açıklama

Anahtar Kelimeler

Apoptosis, inflammation, iNOS, Nf-kappa beta, pulmonary contusion, Acute Lung Injury, Chest Trauma, Oxidative Stress, Nitric-Oxide, Dexamethasone, Rats, Inhibition, Cape

Kaynak

Ulusal Travma Ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery

WoS Q Değeri

Q4

Scopus Q Değeri

Q2

Cilt

25

Sayı

5

Künye