Caffeic acid phenethyl ester ameliorates pulmonary inflammation and apoptosis reducing Nf-?? activation in blunt pulmonary contusion model
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Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Turkish Assoc Trauma Emergency Surgery
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
BACKGROUND: Pulmonary contusion (PC) is an important life-threatening clinical condition characterized by lung injury and inflammation. Caffeic acid phenethyl ester (CAPE) is a biological agent with potent antioxidant and anti-inflammatory effects. This study aimed to investigate the potential effects of CAPE on tissue damage, nuclear factor kappa-beta (Nf-kappa beta) activity, inducible nitric oxide synthase (iNOS) synthesis, and pulmonary apoptosis in an experimental PC model. METHODS: Forty adult Wistar albino rats were used in this study and divided into four groups as follows: control, PC, PC + CAPE, and CAPE. CAPE was administered intraperitoneally for seven days following PC formation (10 jimol/kg, dissolved in dimethyl sulfoxide). Wet/dry weight ratio in lung tissue was determined. The pulmonary tissue was examined using hematoxylin-eosin and Masson's trichrome histochemical staining and also by scanning electron microscopy. Nf-kappa beta and iNOS activities in the lungs were determined by the indirect immunohistochemical method. Pulmonary apoptosis was detected by the TUNEL method. RESULTS: Increased leukocyte infiltration score, pulmonary edema, alveolar damage, and increased Nf-kappa beta and iNOS activities were determined in the PC group. CAPE administration inhibited Nf-kappa beta and iNOS activities and pulmonary apoptosis. CONCLUSION: In this study, the findings showed that CAPE inhibited tissue damage by suppressing inflammatory mediators of Nf-kappa beta and iNOS activities. Also, CAPE was found to be protective in the lung tissue and could be used as a therapeutic agent.
Açıklama
Anahtar Kelimeler
Apoptosis, inflammation, iNOS, Nf-kappa beta, pulmonary contusion, Acute Lung Injury, Chest Trauma, Oxidative Stress, Nitric-Oxide, Dexamethasone, Rats, Inhibition, Cape
Kaynak
Ulusal Travma Ve Acil Cerrahi Dergisi-Turkish Journal of Trauma & Emergency Surgery
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
25
Sayı
5