Royal jelly attenuates gastric mucosal injury in a rat ethanol-induced gastric injury model
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Science and Business Media B.V.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of the study was to investigate traditionally used Royal Jelly (RJ) for treating an ethanol-induced gastric ulcer model in rats. A total of 32 Wistar albino male rats were divided into 4 groups of 8: group I = Control, group II = Ethanol, group III = RJ + Ethanol, and group IV = Lansoprazole + Ethanol. In groups II, III, and IV, animals were administered 1 ml of absolute ethanol orally after a 24-h fast to induce ulcer formation. The histopathological changes in the gastric mucosa were determined using hematoxylin-eosin (H&E) staining. Immunohistochemically, inducible nitric oxide (iNOS) and nuclear factor kappa beta (Nf-??) markings were evaluated in gastric tissue. Cell death in the gastric mucosa was determined by the TUNEL method. Oxidative status markers, superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT), and myeloperoxidase (MPO) levels were determined spectrophotometrically. Expression of the interleukin – 1 beta (IL-1?) and tumor necrosis factor-? (TNF-?) genes in gastric tissues was determined by real-time PCR; and TNF-?, IL-10, and IL-1? levels were determined. RJ was found to inhibit iNOS and Nf-?? activity in the gastric mucosa and prevent epithelial cell apoptosis. In particular, pro-inflammatory cytokines TNF-? and IL-1? levels were significantly decreased in the RJ + Ethanol group compared to the Ethanol group. In addition, a decrease in the MPO level indicated that RJ prevented tissue damage, especially by preventing inflammatory cell infiltration. The study demonstrated a possible gastroprotective effect of RJ in a rat ethanol-induced gastric ulcer model. © 2020, Springer Nature B.V.
Açıklama
Anahtar Kelimeler
Apoptosis, Gastric ulcer, Inducible nitric oxide synthase, Nuclear factor-kappa beta, Royal Jelly, catalase, immunoglobulin enhancer binding protein, inducible nitric oxide synthase, interleukin 10, interleukin 1beta, lansoprazole, malonaldehyde, myeloperoxidase, royal jelly, superoxide dismutase, tumor necrosis factor, alcohol, catalase, central depressant agent, cytokine, fatty acid, immunoglobulin enhancer binding protein, malonaldehyde, royal jelly, superoxide dismutase, adult, animal experiment, animal model, animal tissue, apoptosis, Article, cell death, controlled study, enzyme activity, enzyme linked immunosorbent assay, epithelium cell, ethanol-induced gastric ulcer, gene expression, hematoxylin eosin staining, histopathology, IL 1beta gene, immunohistochemistry, male, nonhuman, protein blood level, protein expression, rat, real time polymerase chain reaction, spectrophotometry, staining, stomach protection, TNF alpha gene, transcription initiation, TUNEL assay, Wistar rat, animal, disease model, drug effect, genetics, injury, metabolism, oxidative stress, stomach mucosa, stomach ulcer, Animals, Apoptosis, Catalase, Central Nervous System Depressants, Cytokines, Disease Models, Animal, Ethanol, Fatty Acids, Gastric Mucosa, Gene Expression, Male, Malondialdehyde, NF-kappa B, Oxidative Stress, Rats, Wistar, Stomach Ulcer, Superoxide Dismutase
Kaynak
Molecular Biology Reports
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
47
Sayı
11
