Evaluation of Variation on Myostatin (MSTN) Gene of Turkish Donkey Populations in Thrace Region of Turkey

Basit Öğe Kaydı
dc.authoridISIK, RAZIYE/0000-0003-2982-6562
dc.authorscopusid57200696504
dc.authorscopusid26028615800
dc.authorscopusid57728408100
dc.authorwosidISIK, RAZIYE/V-4030-2017
dc.authorwosidISIK, RAZIYE/O-2454-2019
dc.contributor.authorIşık, Raziye
dc.contributor.authorÖzdil, Fulya
dc.contributor.authorMeral, Sena
dc.date.accessioned2023-04-20T08:02:28Z
dc.date.available2023-04-20T08:02:28Z
dc.date.issued2022
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractThe study aimed to determine the MSTN gene variation in 90 donkeys reared in the Thrace region of Turkey. Myostatin (MSTN), also named GDF-8 (growth differentiation factor 8) is a part of the transforming growth factor beta (TGF-beta) superfamily and it has a negative regulator role on muscle mass, growth and development in mammalian species. MSTN gene regulates the skeletal muscle growth in a negative way and has a significant role in homeostasis of skeletal muscles. Also, in muscle fibers balance of protein has been promoted by Myostatin factor. The total of 866 bp long partial intron 1 and 2, whole exon 2 regions of MSTN gene was amplified and PCR products analysed using DNA sequencing. In this study, a novel synonymous SNP was determined as g.4183919 G>A in the second exon region of the MSTN gene which does not cause an amino acid change in the protein. The G>A transition caused a silent mutation in leucine (leu) amino acid. Alterations in mRNA level and functionality of protein can occur due to synonymous mutations. Since leucine is an important amino acid that can avoid muscle mass loss and inhibits the expression of Myostatin, it can be said that silent mutation of Leu in donkeys may have altered the muscle mass and physical factor of donkeys in this study. Mutant leucine may have a lower efficient effect on preventing loss of muscles and causes more Myostatin protein expression. The identified SNP was firstly released and the DNA sequences of the MSTN gene in Turkish donkeys was revealed for the first time with recent study. Turkish donkeys lacked these mutations that were identified before in horses, which cause for the less might require for race ability of donkeys. The sequences of MSTN gene were submitted to the NCBI GenBank with the accession number: MW970078- MW970079. Further studies are needed to conduct, on protein and molecular levels, SNPs on the MSTN gene and their association with the morphological characters that may affect economic traits in donkey breeds.
dc.identifier.doi10.33462/jotaf.1015587
dc.identifier.endpage434
dc.identifier.issn1302-7050
dc.identifier.issn2146-5894
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85131350934
dc.identifier.scopusqualityQ3
dc.identifier.startpage426
dc.identifier.trdizinid1152658
dc.identifier.urihttps://doi.org/10.33462/jotaf.1015587
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10949
dc.identifier.volume19
dc.identifier.wosWOS:000813467900017
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorIşık, Raziye
dc.institutionauthorÖzdil, Fulya
dc.language.isoen
dc.publisherUniv Namik Kemal
dc.relation.ispartofJournal of Tekirdag Agriculture Faculty-Tekirdag Ziraat Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMstn
dc.subjectDonkey
dc.subjectThrace
dc.subjectSnp
dc.subjectSequencing
dc.subjectHorse
dc.subjectMutations
dc.subjectIdentification
dc.subjectPolymorphisms
dc.subjectAssociation
dc.subjectVariants
dc.subjectTraits
dc.subjectCattle
dc.subjectPhenotype
dc.subjectDeletion
dc.titleEvaluation of Variation on Myostatin (MSTN) Gene of Turkish Donkey Populations in Thrace Region of Turkey
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
10949.pdf
Boyut:
1.36 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
Tekirdağ Ziraat Fakültesi Dergisi
TR-Dizin İndeksli Yayınlar Koleksiyonu
Ziraat Fakültesi Koleksiyonu