Determination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected from Western Turkey

Basit Öğe Kaydı
dc.authorscopusid57189634048
dc.authorscopusid57216389734
dc.authorscopusid57192573046
dc.authorscopusid57190797212
dc.authorscopusid57225437022
dc.authorwosidTuna, Metin/ABA-4295-2020
dc.contributor.authorSavaş Tuna, Gülsemin
dc.contributor.authorDuyu, Gözde
dc.contributor.authorUzun, Kardelen
dc.contributor.authorYücel, Gülru
dc.contributor.authorTuna, Metin
dc.date.accessioned2022-05-11T14:14:23Z
dc.date.available2022-05-11T14:14:23Z
dc.date.issued2017
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractHypericum perforatum L. (St John's Wort) is a medicinal plant that produces pharmaceutically important compounds with antidepressive and anticancer activities. H. perforatum is a facultative apomictic species as it has the ability to reproduce with multiple reproduction mechanisms affecting genetic structure and chemical composition of the plants. The objective of this study was to determine nuclear DNA contents and ploidy levels of H. perforatum L. plants growing naturally in the flora of Turkey. The seeds of 39 Hypericum perforatum L. accessions collected from 23 different locations in Turkey were used in the study. Nuclear DNA contents of three different seedlings for each of the 39 H. perforatum accessions were determined using flow cytometry. Based on the results of flow cytometric analysis, nuclear DNA contents of the accessions varied between 0.8-2.57 pg 2C(-1). Nuclear DNA content differences observed among H. perforatum accessions were statistically significant (P<0.01) and the Duncan test revealed that the accessions formed three clearly distinguishable main groups. Mean nuclear DNA contents of each group were 0.80 pg 2C(-1), 1.58 pg 2C(-1) (1.36-1.73 pg 2C(-1)) and 2.38 pg 2C(-1) (2.15-2.57 pg 2C(-1)). Ploidy levels were determined by correlating nuclear DNA content of accessions with the number of their mitotic chromosomes by counting chromosome number of one plant from each group. Findings suggest that H. perforatum accessions used in this study had the following three different ploidy levels: diploid, tetraploid and hexaploid. The percentages of diploid, tetraploid and hexaploid accessions were 2.2, 86.4 and 11.4% respectively.
dc.identifier.doi10.15832/ankutbd.385863
dc.identifier.endpage403
dc.identifier.issn2148-9297
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85038883797
dc.identifier.scopusqualityN/A
dc.identifier.startpage395
dc.identifier.urihttps://doi.org/10.15832/ankutbd.385863
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5887
dc.identifier.volume23
dc.identifier.wosWOS:000418281100003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorYücel, Gülru
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherAnkara Univ, Fac Agr
dc.relation.ispartofJournal of Agricultural Sciences-Tarim Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHypericum perforatum
dc.subjectFlow cytometry
dc.subjectNuclear DNA content
dc.subjectPloidy level
dc.subjectMedicinal plants
dc.subjectGenome Size
dc.subjectIntraspecific Variation
dc.subjectFlow-Cytometry
dc.subjectVariability
dc.subjectPopulations
dc.subjectEvolution
dc.subjectPlants
dc.titleDetermination of Nuclear DNA Content and Ploidy of Hypericum perforatum L. Accessions Collected from Western Turkey
dc.title.alternativeTürkiye'nin batı bölgelerinden toplanmış Hypericum perforatum L. aksesyonlarının çekirdek DNA içerikleri ile ploidi düzeylerinin belirlenmesi]
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
5887.pdf
Boyut:
1.58 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Fen Edebiyat Fakültesi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu
Ziraat Fakültesi Koleksiyonu