Treatment with Melatonin Enhances the Embryo Quality and the Development of Vitrified/warmed Eight Cell Mouse Embryos by Solid Surface Vitrification (SSV)
Yükleniyor...
Dosyalar
Tarih
2020
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
NLM (Medline)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
BACKGROUND: Melatonin is an endocrine hormone secreted from the pineal gland located outside the blood-brain barrier. OBJECTIVE: In this study, in vitro propagated eight-cell mouse embryos were vitrified by the Solid Surface Vitrification (SSV) method and after thawing, their in vitro development and embryo qualities in melatonin added media were investigated. METHODS: Pronuclear stage embryos obtained from super ovulated B6CBAF1/J strain mice, were cultured until the eight-cell stage. Then these eight-cell embryos were vitrified by the SSV method and after thawing, cultured in melatonin added media at 37°C and 5 %CO2 conditions until the blastocyst stage. RESULT: In the experimental period, in vitro embryo development rates of the control, SSV and +10-12 M melatonin groups were observed as 97%, 86% and 93%, respectively. CONCLUSION: Our results indicated that melatonin addition slightly increased the development rates and total cell numbers of embryos vitrified by the SSV method.
Açıklama
Anahtar Kelimeler
melatonin, animal, blastocyst, cryopreservation, mammalian embryo, mouse, vitrification, Animals, Blastocyst, Cryopreservation, Embryo, Mammalian, Melatonin, Mice, Vitrification
Kaynak
Cryo letters
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
41
Sayı
2
