Genotyping of the ? – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses

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dc.contributor.authorKaradağ, Orhan
dc.contributor.authorKader Esen, Vasfiye
dc.contributor.authorYüksel, Mehmet Akif
dc.contributor.authorKüçükkebabçı, Mustafa
dc.contributor.authorSevim, Semih
dc.contributor.authorÇetin, İlkay
dc.contributor.authorKaptan, Binnur
dc.contributor.authorSoysal, Mehmet İhsan
dc.date.accessioned2023-05-06T17:19:35Z
dc.date.available2023-05-06T17:19:35Z
dc.date.issued2023
dc.departmentFakülteler, Ziraat Fakültesi, Gıda Mühendisliği Bölümü
dc.departmentFakülteler, Ziraat Fakültesi, Zootekni Bölümü
dc.description.abstractThe aim of this study was to investigate the polymorphism of the ? – casein gene (kappa casein, CSN3) in buffaloes and determine its effects on milk yield traits. Data were obtained from 2016 to 2019 from all animals in the ongoing Anatolian Buffalo Breeding Project. Genomic DNA was collected from a total of 209 buffaloes of different ages and sexes, namely 31 Murrah, 46 Anatolian buffaloes, and 132 of their crosses (Murrah × Anatolian). Genotyping of CSN3 gene by polymerase chain reaction–restriction fragment length polymorphism (PCR – RFLP) technique with restriction enzyme (AcuI) revealed three genotypes: AA (0.689), AB (0.282), and BB (0.028), and the frequencies of A and B alleles were 0.83 and 0.17, respectively. A total of 113 milk yield data and 74 milk component analyses of dairy buffaloes were used to compare CSN3 genotypes for milk yield traits. Statistical analysis showed that there was a significant difference in lactation milk yield between CSN3 genotypes: BB, 1560.3 ± 326.7 kg, AB, 1278.1 ± 111.3 kg, and AA, 1060.9 ± 85.6 kg (p < 0.05). However, the mean values of lactation length and milk components were not significant between genotypes (p > 0.05). These results suggest that these variations $(135^{ThrACC} / IleATC /136^{ThrACC} / ThrACT)$ in the buffalo CSN3 gene may be used as genetic markers in dairy buffalo breeding programs.
dc.identifier.doi10.55730/1300-0128.4268
dc.identifier.endpage61
dc.identifier.issn1300-0128
dc.identifier.issn1303-6181
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85158885713
dc.identifier.scopusqualityQ3
dc.identifier.startpage55
dc.identifier.urihttps://doi.org/10.55730/1300-0128.4268
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1159677
dc.identifier.urihttps://hdl.handle.net/20.500.11776/11874
dc.identifier.volume47
dc.identifier.wosWOS:000971877800007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKaptan, Binnur
dc.institutionauthorSoysal, Mehmet İhsan
dc.language.isoen
dc.relation.ispartofTurkish Journal of Veterinary and Animal Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleGenotyping of the ? – casein gene by PCR – RFLP (AcuI digestion) and its relationship to milk yield traits in Anatolian buffaloes, Murrah buffaloes, and their crosses
dc.typeArticle

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