Development and validation of a sensitive spectrofluorimetric method for the determination of amoxapine in human plasma and urine
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Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A selective and sensitive spectrofluorimetric method was developed and validated for the determination of amoxapine in human plasma and urine. The developed method is based on labeling with 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl chloride) and monitoring at 397nm (excitation)/514nm (emission). The method was validated for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The calibration curves were linear over a concentration range of 250-2500 and 50-1250ng/mL for plasma and urine, respectively. The LOD values were calculated to be 13.31 and 13.17ng/mL for plasma and urine, respectively. The proposed method was applied to study of amoxapine in human plasma and urine. Copyright (c) 2013 John Wiley & Sons, Ltd.
Açıklama
Anahtar Kelimeler
derivatization, spectrofluorimetry, plasma, urine, validation, dansyl chloride, Performance Liquid-Chromatography, Tricyclic Antidepressants, Whole-Blood, Metabolites, Chloride, Serum
Kaynak
Luminescence
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
29
Sayı
3
