Investigation of the antibacterial and anticarcinogenic effects of Inula viscosa methanol and hexane extracts

Basit Öğe Kaydı
dc.authorscopusid24483063300
dc.authorscopusid57672717400
dc.authorscopusid57672717500
dc.contributor.authorErdal, Berna
dc.contributor.authorYılmaz, Bahar
dc.contributor.authorBaylan, Bensu
dc.date.accessioned2023-04-20T08:05:56Z
dc.date.available2023-04-20T08:05:56Z
dc.date.issued2022
dc.departmentFakülteler, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı
dc.departmentEnstitüler, Sağlık Bilimleri Enstitüsü, Tümör Biyolojisi ve İmmünolojisi Ana Bilim Dalı
dc.description.abstractObjective: Inula viscosa is a perennial medicinal plant belonging to the Asteraceae family. This study aimed at investigating the antibacterial and anticarcinogenic activities of methanol and hexane extracts derived from I. viscosa. Methods: The antibacterial activity of different concentrations of I. viscosa methanol (1.56-800 mg/ml) and hexane (0.19-100 mg/ml) extracts was tested using the disc diffusion method. Listeria monocytogenes, Micrococcus luteus, Escherichia coli and Klebsiella pneumoniae were used as standard reference strains. Minimum inhibitory concentration (MIC) values of the extracts were made for each bacteria using the microdilution method. MIC value was determined as the lowest extract concentration at which growth was not observed. Minimum bactericide concentrations (MBC) were determined for all wells after MIC. The anticarcinogenic activity of I. viscosa was examined using the MTT test. MDA-MB-231 and HT-29 cells were stained with acridine orange and propidium iodide at the IC50 values of the extracts to determine the viable, apoptotic and necrotic cells. Results: Zone diameters of the methanol extract for L. monocytogenes, M. luteus, E. coli and K. pneumoniae were 8, 16, 7 and 9 mm, respectively. Zone diameters of the hexane extract for M. luteus, E. coli and K. pneumoniae were 14, 7 and 9 mm, respectively. MIC values of the methanol extract were 25 mg/ml for M. luteus, 200 mg/ml for L. monocytogenes and K. pneumoniae, and 400 mg/ml for E. coli. MIC values of the hexane extract were 400 mg/ml for L. monocytogenes, E. coli ve K. pneumoniae, and 100 mg/ml for M. luteus. MBC values were similar to MIC values. IC50 values for MDA-MB-231 and HT-29 cells were 25 mg/ml and 200 mg/ ml for the methanol extract, and 6.25 mg/ml and 12.5 mg/ml for the hexane extract, respectively. Depending on increased concentration levels, the presence of necrotic and early apoptotic cells was observed among MDA-MB-231 and HT-29 cells. Conclusion: Methanol and hexane extracts of I. viscosa were found to have antibacterial effects on the studied bacteria and anticarcinogenic activity on MDA-MB-231 and HT-29 cells. Conducting further studies to demonstrate the effects of these extracts on different cancer cell lines and bacterial strains will contribute to the field of complementary medicine. © 2022. All Rights Reserved.
dc.identifier.doi10.5505/TurkHijyen.2022.55798
dc.identifier.endpage144
dc.identifier.issn0377-9777
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85129763248
dc.identifier.scopusqualityQ4
dc.identifier.startpage133
dc.identifier.trdizinid1119066
dc.identifier.urihttps://doi.org/10.5505/TurkHijyen.2022.55798
dc.identifier.urihttps://hdl.handle.net/20.500.11776/11116
dc.identifier.volume79
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorErdal, Berna
dc.institutionauthorYılmaz, Bahar
dc.institutionauthorBaylan, Bensu
dc.language.isoen
dc.publisherRefik Saydam National Public Health Agency (RSNPHA)
dc.relation.ispartofTurk Hijyen ve Deneysel Biyoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectantibacterial
dc.subjectAntibakteriyel
dc.subjectanticarcinogenic
dc.subjectAntikanserojenik
dc.subjectInula viscosa
dc.subjectInula viscosa
dc.titleInvestigation of the antibacterial and anticarcinogenic effects of Inula viscosa methanol and hexane extracts
dc.title.alternativeInula viscosa metanol ve hekzan ekstraktlarının antibakteriyel ve antikanserojenik etkilerinin incelenmesi
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
11116.pdf
Boyut:
1.32 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

Scopus İndeksli Yayınlar Koleksiyonu
Sağlık Bilimleri Enstitüsü Diğer Koleksiyonu
Tıp Fakültesi Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu