Investigations on the role of cuticular wax in resistance to powdery mildew in grapevine
Yükleniyor...
Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer Japan Kk
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Cuticular wax on the plant epidermis inhibits or enhances prepenetration events of powdery mildew (Erysiphe necator Schwein). We examined the role of cuticular leaf and berry waxes as a resistance mechanism in four grapevine genotypes (Italia x Mercan-174, Gurcu, Isabella, A-zer KarasA +/-) resistant to powdery mildew after natural infection and inoculation. To understand cuticular wax properties, we determined the amount of wax and antifungal effects of thin layer chromatography (TLC) fractions from cuticular leaf and berry waxes, then assessed the chemical composition of fractions with different antifungal activities using gas chromatography/mass spectrometry (GC/MS). Susceptible genotypes Cabernet Sauvignon and Italia were used for comparison. Resistant and sensitive genotypes did not differ significantly in the total amount of wax on leaves and berries; however, 27 fatty acids, 26 alkanes, 6 terpenes, 4 indole derivatives and 4 ketones, and 3 amides, 3 phenols and 3 steroids were detected in fractions with high antifungal activity (ae<yen>75% inhibition of germination) in leaf and/or berry cuticular waxes of resistant genotypes only. These compounds may be evaluated as markers for powdery mildew resistance during genotype selection in a grapevine breeding program.
Açıklama
Anahtar Kelimeler
Antifungal compounds, Cuticular wax, Erysiphe necator, Grapevine powdery mildew, Prepenetration Processes, Blumeria-Graminis, Barley, Arabidopsis, Fungus, Leaves
Kaynak
Journal of General Plant Pathology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
83
Sayı
5
