Magnetite nanoparticles-based hydroxyl radical scavenging activity assay of antioxidants using N, N-dimethyl-p-phenylenediamine probe

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dc.authorscopusid37025577800
dc.authorscopusid57210474027
dc.authorscopusid55639537800
dc.authorscopusid6508161441
dc.authorscopusid57219942244
dc.contributor.authorCan, Z.
dc.contributor.authorKeskin, B.
dc.contributor.authorArda, A.
dc.contributor.authorErçağ, Erol
dc.contributor.authorApak, M.R.
dc.date.accessioned2022-05-11T14:31:04Z
dc.date.available2022-05-11T14:31:04Z
dc.date.issued2020
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractExcessive amounts of reactive oxygen species (ROS), unless counterbalanced by antioxidants, can cause cellular damage under oxidative stress conditions; therefore, antioxidative defenses against ROS must be measured. With the development of nanotechnology, nanoparticles have found numerous applications in science, health, and industries. Magnetite nanoparticles (Fe3O4:MNPs) have attracted attention because of their peroxidase-like activity. In this study, hydroxyl radicals (·OH) generated by MNPs-catalyzed degradation of H2O2 converted the N,N-dimethyl-p-phenylenediamine (DMPD) probe into its colored DMPD·+ radical cation, which gave an absorbance maximum at ? = 553 nm. In the presence of antioxidants, ·OH was partly scavenged by antioxidants and produced less DMPD·+, causing a decrease in the 553 nm-absorbance. Antioxidant concentrations were calculated with the aid of absorbance differences between the reference and sample solutions. The linear working ranges and trolox equivalent antioxidant capacity coefficients of different classes of antioxidants were determined by applying the developed method. In addition, binary and ternary mixtures of antioxidants were tested to observe the additivity of absorbances of mixture constituents. The method was applied to real samples such as orange juice and green tea. Student t-test, F tests, and the Spearman's rank correlation coefficient were used for statistical comparisons. © 2020 TUBITAK. All rights reserved.
dc.description.sponsorship28963
dc.description.sponsorshipThis study was funded by İstanbul University-Cerrahpaşa with the project number 28963. We express our gratitude to İstanbul University-Cerrahpaşa Application and Research Center for the Measurement of Food Antioxidants (GAAM) for assay development.
dc.description.sponsorshipThis study was funded by Istanbul University-Cerrahpa?a with the project number 28963. We express our gratitude to Istanbul University-Cerrahpa?a Application and Research Center for the Measurement of Food Antioxidants (GAAM) for assay development.
dc.identifier.doi10.3906/KIM-2006-9
dc.identifier.endpage1375
dc.identifier.issn1300-0527
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85096191552
dc.identifier.scopusqualityQ3
dc.identifier.startpage1366
dc.identifier.urihttps://doi.org/10.3906/KIM-2006-9
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7303
dc.identifier.volume44
dc.indekslendigikaynakScopus
dc.institutionauthorErçağ, Erol
dc.language.isoen
dc.publisherTUBITAK
dc.relation.ispartofTurkish Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAntioxidant activity
dc.subjectColorimetric probe
dc.subjectMagnetite (Fe3O4) nanoparticles
dc.subjectN,N-Dimethyl-p-phenylenediamine
dc.subjectReactive oxygen species (ROS)
dc.titleMagnetite nanoparticles-based hydroxyl radical scavenging activity assay of antioxidants using N, N-dimethyl-p-phenylenediamine probe
dc.typeArticle

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