Magnetite nanoparticles-based hydroxyl radical scavenging activity assay of antioxidants using N, N-dimethyl-p-phenylenediamine probe
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
TUBITAK
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Excessive amounts of reactive oxygen species (ROS), unless counterbalanced by antioxidants, can cause cellular damage under oxidative stress conditions; therefore, antioxidative defenses against ROS must be measured. With the development of nanotechnology, nanoparticles have found numerous applications in science, health, and industries. Magnetite nanoparticles (Fe3O4:MNPs) have attracted attention because of their peroxidase-like activity. In this study, hydroxyl radicals (·OH) generated by MNPs-catalyzed degradation of H2O2 converted the N,N-dimethyl-p-phenylenediamine (DMPD) probe into its colored DMPD·+ radical cation, which gave an absorbance maximum at ? = 553 nm. In the presence of antioxidants, ·OH was partly scavenged by antioxidants and produced less DMPD·+, causing a decrease in the 553 nm-absorbance. Antioxidant concentrations were calculated with the aid of absorbance differences between the reference and sample solutions. The linear working ranges and trolox equivalent antioxidant capacity coefficients of different classes of antioxidants were determined by applying the developed method. In addition, binary and ternary mixtures of antioxidants were tested to observe the additivity of absorbances of mixture constituents. The method was applied to real samples such as orange juice and green tea. Student t-test, F tests, and the Spearman's rank correlation coefficient were used for statistical comparisons. © 2020 TUBITAK. All rights reserved.
Açıklama
Anahtar Kelimeler
Antioxidant activity, Colorimetric probe, Magnetite (Fe3O4) nanoparticles, N,N-Dimethyl-p-phenylenediamine, Reactive oxygen species (ROS)
Kaynak
Turkish Journal of Chemistry
WoS Q Değeri
Scopus Q Değeri
Q3
Cilt
44
Sayı
5
