Spectrofluorimetric determination of nateglinide in pure and pharmaceutical preparations through derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole
Yükleniyor...
Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pleiades Publishing Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A sensitive method was developed for the determination of nateglinide in pure and pharmaceutical preparations. The proposed method is based on the derivatization reaction between nateglinide and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole in borate buffer of pH 10. Fluorescent intensity of derivative was measured at 537 nm using an excitation wavelength of 464 nm. The described method was validated and the analytical parameters of linearity, limit of detection, limit of quantification, accuracy, precision (intra- and inter-day) and recovery were evaluated. The assay was linear over the concentration range of 50-500 ng/mL. The proposed method was applied to study of nateglinide in pure and pharmaceutical preparations.
Açıklama
Anahtar Kelimeler
Performance Liquid-Chromatography, Precolumn Derivatization, Human Serum, Human Plasma, Hplc, 4-Chloro-7-Nitrobenzofurazan, Agent
Kaynak
Optics and Spectroscopy
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
116
Sayı
1
