Leptin Receptor Gene Polymorphisms in Some Turkish Donkey Populations
Yükleniyor...
Dosyalar
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
NLM (Medline)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Leptin receptor is a fundamental regulator in physiological functions of the regulation of food intake, energy homeostasis, immune function, and reproduction as well as on ovarian follicular cells on the placenta and lactating mammary glands. The aim of this study was to investigate the LEPR gene polymorphism in 60 donkeys reared in Thrace region of Turkey. A 585 bp long partial intron 6, exon 7, intron 7, and exon 8 regions of LEPR gene were amplified, and polymerase chain reaction products analyzed via DNA sequencing. A novel single-nucleotide polymorphism (SNP) was identified as g.713668A>G in the seventh exon region of LEPR gene. This novel SNP was first identified, and the partial DNA sequence of LEPR gene in donkeys was reported for the first time in this study, and these sequences were deposited to NCBI Genbank database with the accession number: MK807114-MK807115. The A>G transition revealed a silent mutation (CAA-CAG) in glutamine amino acid. This nucleotide mutation could cause the changes of secondary structure of protein and expression level of LEPR hormone. For this reason, additional studies are needed to reveal new SNPs and in the LEPR gene that may affect economic traits and structure of protein in donkey breeds. Copyright © 2019 Elsevier Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
DNA sequencing, Donkey, Equus asinus, LEPR gene, Turkey, leptin receptor, animal, Equidae, female, genetics, lactation, pregnancy, single nucleotide polymorphism, turkey (bird), Animals, Equidae, Female, Lactation, Polymorphism, Single Nucleotide, Pregnancy, Receptors, Leptin, Turkey
Kaynak
Journal of equine veterinary science
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
84
