Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue

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Küçük Resim

Tarih

2018

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Spandidos Publ Ltd

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Non-steroidal anti-inflammatory drugs (NSAIDs) that are applied through oral, injectable or topical routes have been widely used in painful and inflammatory musculoskeletal diseases. The current study aimed to determine whether naproxen, an aryl acetic acid derivative with analgesic and anti-inflammatory effects, has a toxic effect on human chondrocytes. Samples containing monolayer primary chondrocyte cultures were prepared following resection from osteochondral tissues obtained from patients with gonarthrosis. Cell viability, toxicity and proliferation and levels of stage-specific embryonic antigen-1, a precursor to human prechondrocytes, were evaluated spectrophotometrically. The results from the untreated control group were compared with those of the study groups, where naproxen was administered in varying doses (1-1,000 mu M). Surface morphologies of the cells were compared using inverted light and environmental scanning electron microscopy. Treatment groups were compared by analysis of variance with Tukey's honest difference post hoc test. P<0.01 was considered to indicate a statistically significant difference. The research revealed significant changes to proliferation and differentiation of chondrocytes in all treatment groups (P<0.01). Naproxen was demonstrated to suppress chondrocyte proliferation and differentiation, which may be an important factor to consider when prescribing this medication to patients.

Açıklama

Anahtar Kelimeler

aryl acetic acid, chondrotoxicity, proliferation, stage-specific embryonic antigen-1, Mesenchymal Stem-Cells, Blood-Pressure, In-Vitro, Human Chondrocytes, Synovial-Fluid, Nsaids, Transport, Arthritis, Ibuprofen, Membrane

Kaynak

Experimental and Therapeutic Medicine

WoS Q Değeri

Q4

Scopus Q Değeri

N/A

Cilt

16

Sayı

3

Künye