The Potential of Microarray Databases to Identify Tissue Specific Genes
Yükleniyor...
Dosyalar
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kafkas Univ, Veteriner Fakultesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Tissue specific genes play important roles in development and metabolism. Currently, GenBank has 3363628 GEO Profiles and 397988 microarray data related to various tissues. To evaluate the huge amounts of data and identify tissue specific genes, it is necessary to develop and use new strategies. To that end, this study discusses if microarray and microarray related GEO Profiles are a useful tool to identify new tissue specific genes. In the current study, adipose tissue sellected as a target tissue in order to find new tissue specific genes. Therefore, the human and mouse microarray data were analyzed comparatively. To support the microarray data, adipose tissue related GEO Profiles were selected from PubMed. Subsequently, adipose tissue related microarray and GEO Profiles were analysed simultaneously. According to analysis of microarray and GEO Profiles, Chrdl1 (Chordin-like 1) gene was hypothesized as a novel adipose specific gene. In order to test the hypothesis, RT-PCR analysis were performed for the bovine tissue distribution. As a result, the hypothesis was successfully tested and Chrdl1 gene was found highly specific in bovine adipose tissue than in various other tissues. Thus, it is concluded that microarray and microarray related GEO Profiles are a useful tool to identify new tissue specific genes.
Açıklama
Anahtar Kelimeler
Adipose tissue, Adipose specific gene, Chordin-like 1, Microarray, Adipose Triglyceride Lipase, Ppar-Gamma, Nutritional Regulation, 3t3-L1 Adipocytes, Expression, Obesity, Adipogenesis, Proteins, Insulin, Cloning
Kaynak
Kafkas Universitesi Veteriner Fakultesi Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
22
Sayı
1