Molecular identification of Eimeria species of broiler chickens in Turkey

Basit Öğe Kaydı
dc.authorid0000-0003-3460-3849
dc.authorscopusid35304911700
dc.authorscopusid36805409600
dc.authorscopusid15769247000
dc.authorscopusid7801695497
dc.authorscopusid6603080345
dc.authorwosidvatansever, zati/A-2344-2016
dc.contributor.authorGüven, Esin
dc.contributor.authorBeckstead, Robert B.
dc.contributor.authorKar, Sırrı
dc.contributor.authorVatansever, Zati
dc.contributor.authorKaraer, Zafer
dc.date.accessioned2022-05-11T14:28:30Z
dc.date.available2022-05-11T14:28:30Z
dc.date.issued2013
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractThe objective of this study was to determine whether seven Eimeria species involved in chicken coccidiosis are present in Turkey and to assess their prevelance in commercial flocks. Litter and faecal samples were collected from 1110 broiler flocks housed in 817 farms (about 12% of all broiler farms in Turkey) between September 2006 and September 2007. Coccidian oocysts were found in 624 (56.2 %) of the samples examined. Species-specific polymerase chain reaction (PCR) and nested PCR tests targeting the internal transcribed spacer-1 (ITS-1) sequences of the genomic rDNA were performed for all seven Eimeria species. The results of species-specific PCR assays confirmed the presence of E. maxima, E. tenella, K acervulina and E. praecox, and nested PCR results showed the presence of E mitis and E. brunetti. Although morphologic and morphometric observations revealed the presence of oocysts resembling E. necatrix; it was not confirmed by species specific or nested PCR. Nucleotide sequences of Turkish Eimeria isolates obtained by sequencing of the PCR products from 6 Eimeria species have been entered into the GenBank sequence database under accession numbers HQ680469 through HQ680474.
dc.description.sponsorshipAnkara University Office of Scientific Research ProjectsAnkara University [2005K120140-9-6]
dc.description.sponsorshipThis research was supported by a grant of Ankara University Office of Scientific Research Projects (project number: 2005K120140-9-6) and summarized from Esin Guven's Ph.D thesis.
dc.identifier.doi10.1501/Vetfak_0000002587
dc.identifier.endpage250
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-84880413229
dc.identifier.scopusqualityQ3
dc.identifier.startpage245
dc.identifier.urihttps://doi.org/10.1501/Vetfak_0000002587
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6848
dc.identifier.volume60
dc.identifier.wosWOS:000334986500004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherAnkara Univ Press
dc.relation.ispartofAnkara Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBroiler
dc.subjectEimeria species
dc.subjectspecies-specific PCR
dc.subjectnested PCR
dc.subjectEpidemiologic Aspects
dc.subjectPrevalence
dc.subjectCoccidiosis
dc.subjectDiagnosis
dc.subjectBiology
dc.subjectSpp.
dc.titleMolecular identification of Eimeria species of broiler chickens in Turkey
dc.title.alternativeTürkiye'de etlik piliçlerde görülen Eimeria türlerinin moleküler olarak belirlenmesi]
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
6848.pdf
Boyut:
1.48 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Fen Edebiyat Fakültesi Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu