Validated spectrofluorimetric method for the determination of tamsulosin in spiked human urine, pure and pharmaceutical preparations
Yükleniyor...
Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-Blackwell
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A novel, sensitive and selective spectrofluorimetric method was developed for the determination of tamsulosin in spiked human urine and pharmaceutical preparations. The proposed method is based on the reaction of tamsulosin with 1-dimethylaminonaphthalene-5-sulfonyl chloride in carbonate buffer pH10.5 to yield a highly fluorescent derivative. The described method was validated and the analytical parameters of linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, recovery and robustness were evaluated. The proposed method showed a linear dependence of the fluorescence intensity on drug concentration over the range 1.22 x 10(-7) to 7.35 x 10(-6)M. LOD and LOQ were calculated as 1.07 x 10(-7) and 3.23 x 10(-7)M, respectively. The proposed method was successfully applied for the determination of tamsulosin in pharmaceutical preparations and the obtained results were in good agreement with those obtained using the reference method. Copyright (c) 2013 John Wiley & Sons, Ltd.
Açıklama
Anahtar Kelimeler
derivatization, human urine, method validation, dansyl chloride, Performance Liquid-Chromatography, Human Plasma, Mass-Spectrometry, Sensitive Method, Hydrochloride
Kaynak
Luminescence
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
29
Sayı
3
