A new methodology for the determination of enzyme activity based on carbon nanotubes and glucose oxidase

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dc.authorscopusid56104725700
dc.authorscopusid6506764918
dc.contributor.authorYeşiller, Gülden
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:29:58Z
dc.date.available2022-05-11T14:29:58Z
dc.date.issued2015
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractIn this research, a novel enzyme activity analysis methodology is introduced as a new perspective for this area. The activity of elastase enzyme, which is a digestive enzyme mostly of found in the digestive system of vertebrates, was determined by an electrochemical device composed of carbon nanotubes and a second enzyme, glucose oxidase, which was used as a signal generator enzyme. In this novel methodology, a complex bioactive layer was constructed by using carbon nanotubes, glucose oxidase and a supporting protein, gelatin on a solid, conductive substrate. The activity of elastase was determined by monitoring the hydrolysis rate of elastase enzyme in the bioactive layer. As a result of this hydrolysis of elastase, glucose oxidase was dissociated from the bioactive layer, and following this the electrochemical signal due to glucose oxidase was decreased. The progressive elastase-catalyzed digestion of the bioactive layer containing glucose oxidase decreased the layer's enzymatic efficiency, resulting in a decrease of the glucose oxidation current as a function of the enzyme activity. The ratio of the decrease was correlated to elastase activity level. In this study, optimization experiments of bioactive components and characterization of the resulting new electrochemical device were carried out. A linear calibration range from 0.0303. U/mL to 0.0729. U/mL of elastase was reported. Real sample analyses were also carried out by the new electrochemical device. © 2015 Elsevier B.V.
dc.description.sponsorship113 Z 678; Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK
dc.description.sponsorshipThis study was supported by The Scientific and Technological Research Council of Turkey (TUBİTAK) by the project number of 113 Z 678.
dc.identifier.doi10.1016/j.jpba.2015.07.037
dc.identifier.endpage259
dc.identifier.issn0731-7085
dc.identifier.pmid26257292
dc.identifier.scopus2-s2.0-84938690873
dc.identifier.scopusqualityQ2
dc.identifier.startpage254
dc.identifier.urihttps://doi.org/10.1016/j.jpba.2015.07.037
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7186
dc.identifier.volume115
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYeşiller, Gülden
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCarbon nanotube
dc.subjectCyclic hematopoiesis
dc.subjectElastase
dc.subjectGlucose oxidase
dc.subjectPulmonary emphysema
dc.subjectcarbon nanotube
dc.subjectelastase
dc.subjectglucose oxidase
dc.subjectcarbon nanotube
dc.subjectglucose oxidase
dc.subjectimmobilized enzyme
dc.subjectpancreatic elastase
dc.subjectArticle
dc.subjectcatalysis
dc.subjectelectric conductivity
dc.subjectelectrochemical analysis
dc.subjectenzyme activity
dc.subjectenzyme analysis
dc.subjectglucose oxidation
dc.subjecthuman
dc.subjectimpedance
dc.subjectmethodology
dc.subjectpriority journal
dc.subjectprotein hydrolysis
dc.subjectsensitivity analysis
dc.subjectsurface property
dc.subjectblood
dc.subjectcalibration
dc.subjectchemistry
dc.subjectdevices
dc.subjectelectrochemical impedance spectroscopy
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjectprocedures
dc.subjectsensitivity and specificity
dc.subjectBiosensing Techniques
dc.subjectCalibration
dc.subjectDielectric Spectroscopy
dc.subjectElectrodes
dc.subjectEnzymes, Immobilized
dc.subjectGlucose Oxidase
dc.subjectHumans
dc.subjectNanotubes, Carbon
dc.subjectPancreatic Elastase
dc.subjectSensitivity and Specificity
dc.titleA new methodology for the determination of enzyme activity based on carbon nanotubes and glucose oxidase
dc.typeArticle

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