A new methodology for the determination of enzyme activity based on carbon nanotubes and glucose oxidase
Yükleniyor...
Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this research, a novel enzyme activity analysis methodology is introduced as a new perspective for this area. The activity of elastase enzyme, which is a digestive enzyme mostly of found in the digestive system of vertebrates, was determined by an electrochemical device composed of carbon nanotubes and a second enzyme, glucose oxidase, which was used as a signal generator enzyme. In this novel methodology, a complex bioactive layer was constructed by using carbon nanotubes, glucose oxidase and a supporting protein, gelatin on a solid, conductive substrate. The activity of elastase was determined by monitoring the hydrolysis rate of elastase enzyme in the bioactive layer. As a result of this hydrolysis of elastase, glucose oxidase was dissociated from the bioactive layer, and following this the electrochemical signal due to glucose oxidase was decreased. The progressive elastase-catalyzed digestion of the bioactive layer containing glucose oxidase decreased the layer's enzymatic efficiency, resulting in a decrease of the glucose oxidation current as a function of the enzyme activity. The ratio of the decrease was correlated to elastase activity level. In this study, optimization experiments of bioactive components and characterization of the resulting new electrochemical device were carried out. A linear calibration range from 0.0303. U/mL to 0.0729. U/mL of elastase was reported. Real sample analyses were also carried out by the new electrochemical device. © 2015 Elsevier B.V.
Açıklama
Anahtar Kelimeler
Carbon nanotube, Cyclic hematopoiesis, Elastase, Glucose oxidase, Pulmonary emphysema, carbon nanotube, elastase, glucose oxidase, carbon nanotube, glucose oxidase, immobilized enzyme, pancreatic elastase, Article, catalysis, electric conductivity, electrochemical analysis, enzyme activity, enzyme analysis, glucose oxidation, human, impedance, methodology, priority journal, protein hydrolysis, sensitivity analysis, surface property, blood, calibration, chemistry, devices, electrochemical impedance spectroscopy, electrode, genetic procedures, procedures, sensitivity and specificity, Biosensing Techniques, Calibration, Dielectric Spectroscopy, Electrodes, Enzymes, Immobilized, Glucose Oxidase, Humans, Nanotubes, Carbon, Pancreatic Elastase, Sensitivity and Specificity
Kaynak
Journal of Pharmaceutical and Biomedical Analysis
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
115
