Electrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3

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dc.authorscopusid55928560800
dc.authorscopusid56029590100
dc.authorscopusid6506764918
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorCanbaz, Mehmet Çetin
dc.contributor.authorŞimşek, Çiğdem Sayikli
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:29:54Z
dc.date.available2022-05-11T14:29:54Z
dc.date.issued2014
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractWe have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3. (C) 2014 Elsevier B.V. All rights reserved.
dc.description.sponsorshipTUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [109T 172]
dc.description.sponsorshipThis work was supported by a grant from TUBITAK (The Scientific and Technological Research Council of Turkey, Project number: 109T 172).
dc.identifier.doi10.1016/j.aca.2014.01.041
dc.identifier.endpage38
dc.identifier.issn0003-2670
dc.identifier.issn1873-4324
dc.identifier.pmid24528841
dc.identifier.scopus2-s2.0-84893642278
dc.identifier.scopusqualityQ1
dc.identifier.startpage31
dc.identifier.urihttps://doi.org/10.1016/j.aca.2014.01.041
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7169
dc.identifier.volume814
dc.identifier.wosWOS:000331556400003
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorCanbaz, Mehmet Çetin
dc.institutionauthorŞimşek, Çiğdem Sayikli
dc.institutionauthorSezgintürk, Mustafa Kemal
dc.language.isoen
dc.publisherElsevier Science Bv
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCancer biomarkers
dc.subjectHER-3
dc.subjectAnti-HER-3
dc.subjectSingle frequency impedance
dc.subjectBiosensor
dc.subjectGrowth-Factor Receptor
dc.subjectBreast-Cancer
dc.subjectExpression
dc.subjectDispersion
dc.subjectAntibody
dc.subjectTherapy
dc.subjectFamily
dc.titleElectrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3
dc.typeArticle

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