Electrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Science Bv
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4 pg mL(-1). Kramers-Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3. (C) 2014 Elsevier B.V. All rights reserved.
Description
Keywords
Cancer biomarkers, HER-3, Anti-HER-3, Single frequency impedance, Biosensor, Growth-Factor Receptor, Breast-Cancer, Expression, Dispersion, Antibody, Therapy, Family
Journal or Series
Analytica Chimica Acta
WoS Q Value
Q1
Scopus Q Value
Q1
Volume
814
