Detection of Cryptosporidium spp. in Humans and Calves Through Nested PCR and Carbol Fuchsin Staining Methods in Ankara, Turkey

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dc.authorid0000-0003-3460-3849
dc.authorscopusid37038429500
dc.authorscopusid15769247000
dc.authorscopusid6603724387
dc.authorscopusid6603080345
dc.authorscopusid6602101761
dc.authorscopusid7801695497
dc.authorwosidvatansever, zati/A-2344-2016
dc.contributor.authorSakarya, Yasemin
dc.contributor.authorKar, Sırrı
dc.contributor.authorTanyüksel, Mehmet
dc.contributor.authorKaraer, Zafer
dc.contributor.authorBabür, Cahit
dc.contributor.authorVatansever, Zati
dc.date.accessioned2022-05-11T14:28:24Z
dc.date.available2022-05-11T14:28:24Z
dc.date.issued2010
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractIn this study, a total of 130 fecal samples, obtained from 98 humans and 32 calves from different hospitals and dairy farms in Ankara, respectively were analyzed with carbol fuchsin staining and nested PCR to determine the presence of Cryptosporidium spp. Nested PCR revealed a total of 13 (10.0%) positive amplification results; 12 (37.5%) were from calves and 1(1.02%) from humans. Oocysts were observed in 8 (6.15%) fecal samples belonging to 7 (21.88%) calves and 1 (1.02%) human by carbol fuchsin staining. Concerning the detection technics, it was concluded that nested PCR can be successfully used in diagnosis of cryptosporidiosis, and although carbol fuchsin staining method has sufficient specificity it is less sensitive, especially in cases characterized with excretion of fewer oocysts.
dc.identifier.endpage980
dc.identifier.issn1300-6045
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-78149476485
dc.identifier.scopusqualityQ3
dc.identifier.startpage977
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6814
dc.identifier.volume16
dc.identifier.wosWOS:000285216200013
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKar, Sırrı
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCryptosporidium
dc.subjectNested PCR
dc.subjectCarbol fuchsin
dc.subjectAnkara
dc.subjectFecal Specimens
dc.subjectOocysts
dc.subjectParvum
dc.titleDetection of Cryptosporidium spp. in Humans and Calves Through Nested PCR and Carbol Fuchsin Staining Methods in Ankara, Turkey
dc.title.alternativeAnkara'da i?nsan ve buza?ılarda Cryptosporidium spp. Varlı?ının nested PCR ve carbol fuchsin boyama yöntemleri ile belirlenmesi]
dc.typeArticle

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