A Biosensor for the Determination of beta-galactosidase Activity: A Different Viewpoint on Biosensors

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dc.authorscopusid6506764918
dc.authorscopusid6603789064
dc.authorwosidDİNÇKAYA, ERHAN/AAG-7096-2021
dc.authorwosidSezgintürk, Mustafa Kemal/AAY-1439-2021
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.contributor.authorDinçkaya, Erhan
dc.date.accessioned2022-05-11T14:29:43Z
dc.date.available2022-05-11T14:29:43Z
dc.date.issued2011
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.description.abstractbeta-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor beta-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. beta-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for beta-galactosidase detection was in the range of 0.0118 U mL(-1) to 0.47 U mL(-1) and a shorter response time of similar to 50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of beta-galactosidase activity.
dc.description.sponsorshipEge UniversityEge University; Scientific Research Foundation [2004 FEN 002]; Scientific and Technical Research Council of Turkiye (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)
dc.description.sponsorshipThe authors are grateful for the financial support of Ege University, Scientific Research Foundation (Grant No. 2004 FEN 002) and The Scientific and Technical Research Council of Turkiye (TUBITAK).
dc.identifier.doi10.3109/10731199.2011.560119
dc.identifier.endpage288
dc.identifier.issn1073-1199
dc.identifier.issue5en_US
dc.identifier.pmid21351849
dc.identifier.scopus2-s2.0-80052751993
dc.identifier.scopusqualityN/A
dc.identifier.startpage281
dc.identifier.urihttps://doi.org/10.3109/10731199.2011.560119
dc.identifier.urihttps://hdl.handle.net/20.500.11776/7108
dc.identifier.volume39
dc.identifier.wosWOS:000294740300003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSezgintürk, Mustafa Kema
dc.language.isoen
dc.publisherInforma Healthcare
dc.relation.ispartofArtificial Cells Blood Substitutes and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbeta-galactosidase
dc.subjectglucose oxidase
dc.subjectbiosensor
dc.subjectenzyme activity
dc.subjectelectropolymerization
dc.subjectAlkaline-Phosphatase
dc.subjectEscherichia-Coli
dc.subjectIn-Vivo
dc.subjectSurfaces
dc.subjectEnzyme
dc.subjectLabel
dc.subjectWater
dc.titleA Biosensor for the Determination of beta-galactosidase Activity: A Different Viewpoint on Biosensors
dc.typeArticle

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