A Biosensor for the Determination of beta-galactosidase Activity: A Different Viewpoint on Biosensors
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
Informa Healthcare
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
beta-galactosidase splits lactose into glucose and galactose. Because of its biotechnological interest, we presented a biosensor system in order to monitor beta-galactosidase activity. Immobilization steps of the biosensor were identified by cyclic voltammograms and electrochemical impedance spectroscopy. beta-galactosidase was voltammetrically detected at about +150 mV (vs. Ag/AgCl) in citrate buffer solution (0.05 M, pH 4.8). The linear response for beta-galactosidase detection was in the range of 0.0118 U mL(-1) to 0.47 U mL(-1) and a shorter response time of similar to 50 s. Our results demonstrated the biosensor's electrochemical properties and analytical characteristics were very useful and effective for monitoring of beta-galactosidase activity.
Description
Keywords
beta-galactosidase, glucose oxidase, biosensor, enzyme activity, electropolymerization, Alkaline-Phosphatase, Escherichia-Coli, In-Vivo, Surfaces, Enzyme, Label, Water
Journal or Series
Artificial Cells Blood Substitutes and Biotechnology
WoS Q Value
Q3
Scopus Q Value
N/A
Volume
39
Issue
5
