Ultrasensitive electrochemical detection of cancer associated biomarker HER3 based on anti-HER3 biosensor
Yükleniyor...
Dosyalar
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The development of a new impedimetric biosensor for the detection of HER3, based on self-assembled monolayers (SAMs) of 4-aminothiophenol on gold electrodes, is reported. Anti-HER3 was used as a biorecognition element for the first time in an impedimetric biosensor. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques were applied to characterize the immobilization process and to detect HER3. To provide the best biosensor response all experimental parameters were optimized. In addition, Kramers-Kronigs transform was also performed on the immobilization and measurement processes successfully. The biosensor had a linear detection range of 0.4-2.4 pg/mL. The chrono-impedance technique to real time monitor the interaction between HER3 and anti-HER3 is also implemented. The biosensor has exhibited good repeatability and reproducibility. To demonstrate the feasibility of the biosensor in practical analysis, the artificial serum samples were experienced. (C) 2013 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
HER3, Anti-HER3, Biosensor, Electrochemical impedance spectroscopy, Cancer biomarker, C-Erbb-3 Protein Expression, Growth-Factor Receptors, Erbb3, Dispersion, Carcinoma, Antibody
Kaynak
Talanta
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
120
