An epoxysilane modified indium tin oxide electrode for the determination of PAK 2: Application in human serum samples

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dc.authorscopusid57209756570
dc.authorscopusid57194273220
dc.authorscopusid6506764918
dc.contributor.authorYılmaz, Nergiz
dc.contributor.authorAydın, Elif Burcu
dc.contributor.authorSezgintürk, Mustafa Kemal
dc.date.accessioned2022-05-11T14:02:58Z
dc.date.available2022-05-11T14:02:58Z
dc.date.issued2019
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Kimya Bölümü
dc.departmentRektörlüğe Bağlı Bölümler, Rektörlük, Bilimsel ve Teknolojik Araştırmalar Uygulama ve Araştırma Merkezi
dc.description.abstractIn this study, a sensitive immunosensor was developed for the first time for p21-activated kinase 2 (PAK 2) detection. In the design of the immunosensor, 3-glycidoxypropyltrimethoxysilane (GPTMS) was utilized as an ITO electrode modification material for anti-PAK 2 antibody immobilization. This molecule had epoxy group, which was reactive to amino groups of antibodies. Anti-PAK 2 antibodies were also used as biomolecules for sensitive interaction for PAK 2 antigen. In the presence of PAK 2 antigens, high impedance signal was observed when the process followed by using Electrochemical Impedance Spectroscopy technique (EIS). Apart from EIS technique, Cyclic Voltammetry (CV), Square Wave Voltammetry (SWV) and Single Frequency Impedance (SFI) techniques were utilized. Microscopic surface characterizations of immunosensor fabrication steps were performed by using Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The immunosensor exhibited good sensitivity and selectivity for PAK 2 antigen detection. A linear calibration curve between EIS response and PAK 2 concentration was obtained in the range of 0.005–0.075 pg/mL with the detection limit of 1.5 fg/mL. It had good repeatability, excellent reproducibility and high stability. Additionally, this immunosensor can be reused by simple application protocol. Furthermore, it had good recovery for PAK 2 antigen detection in human serum samples. The good recovery illustrated that the developed immunosensor was a promising tool for PAK 2 detection in practical applications. © 2019 Elsevier B.V.
dc.description.sponsorshipTürkiye Bilimsel ve Teknolojik AraÅŸtirma Kurumu, TÃœBITAK: 113 Z 678
dc.description.sponsorshipThe financial support from TÜBİTAK ( The Scientific and Technological Research Council of Turkey , Project number: 113 Z 678 ) is gratefully acknowledged.
dc.identifier.doi10.1016/j.aca.2019.02.020
dc.identifier.endpage77
dc.identifier.issn0003-2670
dc.identifier.pmid30947997
dc.identifier.scopus2-s2.0-85062189337
dc.identifier.scopusqualityQ1
dc.identifier.startpage68
dc.identifier.urihttps://doi.org/10.1016/j.aca.2019.02.020
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4558
dc.identifier.volume1062
dc.identifier.wosWOS:000462857200006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorYılmaz, Nergiz
dc.institutionauthorAydın, Elif Burcu
dc.language.isoen
dc.publisherElsevier B.V.
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subject3-Glycidoxypropyltrimethoxysilane (GPTMS)
dc.subjectCancer biomarker
dc.subjectElectrochemical impedance spectroscopy
dc.subjectp21-activated kinases (PAKs)
dc.subjectAntibodies
dc.subjectAntigens
dc.subjectAtomic force microscopy
dc.subjectBody fluids
dc.subjectCyclic voltammetry
dc.subjectElectrodes
dc.subjectEnzymes
dc.subjectImmunosensors
dc.subjectScanning electron microscopy
dc.subjectSpectroscopy
dc.subjectSurface measurement
dc.subjectTin oxides
dc.subject3-Glycidoxypropyltrimethoxysilane
dc.subjectAntibody immobilization
dc.subjectCancer biomarkers
dc.subjectElectrochemical impedance spectroscopy techniques
dc.subjectIndium tin oxide electrodes
dc.subjectLinear calibration curve
dc.subjectP-21 activated kinase
dc.subjectSquare wave voltammetry
dc.subjectElectrochemical impedance spectroscopy
dc.subjectindium tin oxide
dc.subjectp21 activated kinase 2
dc.subject3-glycidoxypropyltrimethoxysilane
dc.subjectindium tin oxide
dc.subjectp21 activated kinase
dc.subjectsilane derivative
dc.subjecttin derivative
dc.subjecttumor marker
dc.subjectantigen detection
dc.subjectArticle
dc.subjectatomic force microscopy
dc.subjectcyclic potentiometry
dc.subjectelectrochemistry
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjecthydroxylation
dc.subjectimpedance spectroscopy
dc.subjectlimit of detection
dc.subjectpriority journal
dc.subjectprotein determination
dc.subjectradioimmunoassay
dc.subjectscanning electron microscopy
dc.subjectblood
dc.subjectchemistry
dc.subjectelectrochemical analysis
dc.subjectelectrode
dc.subjectgenetic procedures
dc.subjectBiomarkers, Tumor
dc.subjectBiosensing Techniques
dc.subjectDielectric Spectroscopy
dc.subjectElectrochemical Techniques
dc.subjectElectrodes
dc.subjectHumans
dc.subjectp21-Activated Kinases
dc.subjectSilanes
dc.subjectTin Compounds
dc.titleAn epoxysilane modified indium tin oxide electrode for the determination of PAK 2: Application in human serum samples
dc.typeArticle

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