In vitro propagation of Silene bolanthoides Quezel, Contandr. & Pamukc. and assessment of genetic stability by flow cytometry

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Küçük Resim

Tarih

2018

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Inst Bioloska Istrazivanja Sinisa Stankovic

Erişim Hakkı

info:eu-repo/semantics/openAccess

Özet

Silene bolanthoides Quezel, Contandr. & Pamukc. is an endemic species from Kazdagi (Mt. Ida), Canakkale-Balikesir, Turkey. In order to develop an efficient shoot regeneration protocol, the leaf, nodal and internodal explants of S. bolanthoides were cultured on Murashige and Skoog (MS) medium containing benzyladenine (BA) alone or in combination with alpha-naphthaleneacetic acid (NAA). The highest number of regenerated shoots (5.75 +/- 0.1) was obtained from nodal explants that were cultured on MS medium with 8.8 mu M BA+0.54 mu M NAA. Regenerated shoots were rooted on MS medium without plant growth regulators (PGRs). Rooted plants (2-3 cm) were separately transferred to pots containing a mixture of peat and perlite (3:1 v/v) and acclimatized successfully in a growth chamber. Genetic stability of the propagated plants was assessed by flow cytometry and cytological analysis. Flow cytometry analysis demonstrated that regenerated plants had 2.61 +/- 0.01 pg nuclear DNA (2C) and seed-derived plants had on average 2.58 +/- 0.02 pg/2C. Cytological analysis showed that the regenerated plants had the same chromosome number as seed-derived plants of S. bolanthoides (2n=24). It was determined that regenerated plants were uniform in chromosome number and had a similar DNA content to the seed-derived ones, indicating that the described efficient shoot regeneration protocol can be applied for ex situ conservation of this species.

Açıklama

Anahtar Kelimeler

catchfly, chromosome, endemic, genetic stability, regeneration, Tissue-Culture, Somatic Embryogenesis, Somaclonal Variation, Micropropagation, Plants, Regeneration, Fidelity, Callus, Rapd, Caryophyllaceae

Kaynak

Archives of Biological Sciences

WoS Q Değeri

Q4

Scopus Q Değeri

Q3

Cilt

70

Sayı

1

Künye