Molecular Analyses of the Genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae) By Pcr Methods

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dc.authorid0000-0003-0340-1195
dc.authorid0000-0002-7706-5801
dc.authorid0000-0003-2041-1205
dc.authorscopusid7004114724
dc.authorscopusid33367486100
dc.authorscopusid7102837994
dc.authorwosidDogan, Musa/AAB-3970-2021
dc.authorwosidCabi, Evren/H-2263-2019
dc.authorwosidDoğan, Musa/AAZ-9004-2020
dc.authorwosidYILMAZ, Remziye/M-8310-2018
dc.contributor.authorYılmaz, Remziye
dc.contributor.authorCabi, Evren
dc.contributor.authorDoğan, Musa
dc.date.accessioned2022-05-11T14:28:31Z
dc.date.available2022-05-11T14:28:31Z
dc.date.issued2014
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractRAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase Chain Reaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some species in the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used in the study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments which have been used as a measure of the degree of relationship between species by means of using the RAPD-PCR results with three primers selected for identifying the genetic similarities. Polymorphic melting profiles have been obtained with Post PCR MCA method using three primers. Genetic similarities are calculated for all the species studied with RAPD-PCR and MCA methods, the dendrograms are obtained with the MVSP (Multi Variate Statistical Package) software using UPGMA (Unweighted Pair Group Method with Arithmetic Averages) and Jaccard's Coefficient. Polymorphism between 18 populations of Eremopyrum and 6 Agropyron populations and within the species are determined by using RAPD-PCR and Post PCR melting curve analysis (MCA) respectively.
dc.description.sponsorshipScientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-TBAG-105 T 171]
dc.description.sponsorshipWe would like to thank Prof. Dr. Meral YUCEL, METU Central Lab., Molecular Biology and Biotechnology R&D Center, for providing all equipments used in this study. We also wish to thank to the Scientific and Technical Research Council of Turkey (TUBITAK-TBAG-105 T 171) for their financial assistance for the study.
dc.identifier.endpage774
dc.identifier.issn0556-3321
dc.identifier.issn2070-3368
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-84902006753
dc.identifier.scopusqualityQ3
dc.identifier.startpage769
dc.identifier.urihttps://hdl.handle.net/20.500.11776/6855
dc.identifier.volume46
dc.identifier.wosWOS:000338626100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorCabi, Evren
dc.language.isoen
dc.publisherPakistan Botanical Soc
dc.relation.ispartofPakistan Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectIntergeneric Hybridization
dc.subjectTribe Triticeae
dc.subjectRapd Markers
dc.subjectTaeniatherum
dc.subjectOrientale
dc.titleMolecular Analyses of the Genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae) By Pcr Methods
dc.typeArticle

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