Molecular Analyses of the Genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae) By Pcr Methods
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Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pakistan Botanical Soc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
RAPD-PCR (Random Amplified Polymorphic DNA Polymerase Chain Reaction) and Post PCR (Polymerase Chain Reaction) Melting Curve Analysis (MCA) have been used to investigate the pattern of genetic variation among some species in the genera Eremopyrum (Ledeb.) Jaub. & Spach and Agropyron Gaertner (Poaceae). Thirteen primers have been used in the study based on the RAPD-PCR and MCA analyses. Each species produced a distinct pattern of DNA fragments which have been used as a measure of the degree of relationship between species by means of using the RAPD-PCR results with three primers selected for identifying the genetic similarities. Polymorphic melting profiles have been obtained with Post PCR MCA method using three primers. Genetic similarities are calculated for all the species studied with RAPD-PCR and MCA methods, the dendrograms are obtained with the MVSP (Multi Variate Statistical Package) software using UPGMA (Unweighted Pair Group Method with Arithmetic Averages) and Jaccard's Coefficient. Polymorphism between 18 populations of Eremopyrum and 6 Agropyron populations and within the species are determined by using RAPD-PCR and Post PCR melting curve analysis (MCA) respectively.
Açıklama
Anahtar Kelimeler
Intergeneric Hybridization, Tribe Triticeae, Rapd Markers, Taeniatherum, Orientale
Kaynak
Pakistan Journal of Botany
WoS Q Değeri
Q3
Scopus Q Değeri
Q3
Cilt
46
Sayı
3