A Study of the Effects of Metformin, a Biguanide Derivative, on Annulus Fibrosus and Nucleus Pulposus Cells

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dc.authorid0000-0002-8084-7855
dc.authorid0000-0003-2003-6337
dc.authorid0000-0002-1224-442X
dc.authorscopusid57194611714
dc.authorscopusid56674583800
dc.authorscopusid35197435300
dc.authorscopusid56769801000
dc.authorscopusid57216912962
dc.authorscopusid7005912992
dc.authorwosidsirin, duygu yasar/AAR-8685-2020
dc.authorwosidOzbek, Hanefi/O-3472-2019
dc.contributor.authorKaya, Yasin Emre
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorYılmaz, İbrahim
dc.contributor.authorŞirin, Duygu Yaşar
dc.contributor.authorAkalan, Hande
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2022-05-11T14:12:15Z
dc.date.available2022-05-11T14:12:15Z
dc.date.issued2020
dc.departmentFakülteler, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Beyin ve Sinir Cerrahisi Ana Bilim Dalı
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.description.abstractAIM: To investigate the effects of metformin, a drug used widely for the treatment of type 2 diabetes mellitus, on human primary cell cultures prepared from uninjured segment of disc material intervertebral disk tissues. MATERIAL and METHODS: Primary cell cultures were prepared using the tissues of six patients (three males and three females) who had undergone lumbar microdiscectomy and sequestrectomy. Untreated samples served as the control group, and metformintreated samples served as the experimental group. All the samples were evaluated using an inverted light microscope, acridine orange/propidium iodide staining (AO/PI), and a fluorescence microscope. The cytostatic and cytotoxic effects of metformin, which was administered to the samples using a commercial MTT assay kit, were also evaluated. The data obtained were statistically assessed, and the alpha significance value was accepted as less than 0.05. In addition, for the groups' changes in the expressions of chondroadherin (CHAD), cartilage oligomeric matrix protein (COMP), interleukin-1 beta (IL-1 beta) matrix metalloproteinase 7 (MMP-7), and matrix metalloproteinase 19 (MMP-19), genes related to the extracellular matrix synthesis and degradation were determined using gene-specific TaqMan Gene Expression Assays. RESULTS: The administration of the drug adversely affected nucleus pulposus (NP)/annulus fibrosus (AF) cells and extracellular matrix-like structures. This was statistically significant (p<0.05). CONCLUSION: Clinicians should not disregard the adverse effects of metformin, which is used widely in clinical practice, on the components of intervertebral disk tissues.
dc.identifier.doi10.5137/1019-5149.JTN.27785-19.3
dc.identifier.endpage441
dc.identifier.issn1019-5149
dc.identifier.issue3en_US
dc.identifier.pmid32239484
dc.identifier.scopus2-s2.0-85085264902
dc.identifier.scopusqualityQ3
dc.identifier.startpage434
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.27785-19.3
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5467
dc.identifier.volume30
dc.identifier.wosWOS:000535739800016
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorKaraarslan, Numan
dc.institutionauthorŞirin, Duygu Yaşar
dc.institutionauthorAkalan, Hande
dc.language.isoen
dc.publisherTurkish Neurosurgical Soc
dc.relation.ispartofTurkish Neurosurgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAnnulus fibrosus
dc.subjectIntervertebral disc tissue
dc.subjectMetformin
dc.subjectNucleus pulposus
dc.subjectPrimary human cell culture
dc.titleA Study of the Effects of Metformin, a Biguanide Derivative, on Annulus Fibrosus and Nucleus Pulposus Cells
dc.typeArticle

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