Molecular cytogenetic characterization of common bean (Phaseolus vulgaris L.) accessions

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dc.authorid0000-0003-2089-2790
dc.authorid0000-0002-4368-0988
dc.authorscopusid57189634048
dc.authorscopusid57190797212
dc.authorscopusid38760988400
dc.authorscopusid57188676588
dc.authorscopusid6507781829
dc.authorscopusid6507409829
dc.authorscopusid57225437022
dc.authorwosidTanyolac, Bahattin/AAD-3762-2019
dc.contributor.authorSavaş Tuna, Gülsemin
dc.contributor.authorYücel, Gülru
dc.contributor.authorKaygısız Aşçıoğul, Tansel
dc.contributor.authorAteş, Duygu
dc.contributor.authorEşiyok, Dursun
dc.contributor.authorTanyolaç, Muhammed Bahattin
dc.contributor.authorTuna, Metin
dc.date.accessioned2022-05-11T14:45:55Z
dc.date.available2022-05-11T14:45:55Z
dc.date.issued2020
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractWith an annual global production of approximately 25 million tons, the common bean (Phaseolus vulgaris L), a member of the genus Phaseolus, is one of the major protein sources used as food for humans. In this study, it was aimed to investigate the genome size of the common bean genetic resource collection (154 common bean accessions) in Turkey by flow cytometry (FCM) and determine whether geographical variables affected the genome size. In addition, the number and distribution of 5S and 45S ribosomal DNA loci were designated by performing a fluorescence in situ hybridization (FISH) analysis in some of the accessions. The FCM analyses revealed that the mean nuclear DNA content of the accessions varied from 1.28 pg2C-1 to 1.55 pg2G1 (mean 1.35 pg2C-1), and the differences between these accessions were statistically significant (P < 0.01). Intraspecific variation in the genome size was determined, and a positive correlation was found between the altitude and genome size. However, latitude and longitude did not have any statistically significant effect on the genome size. In the principal coordinate analysis, the accessions were divided into 3groups. Based on the results of the FISH analysis performed on 5 different accessions with varying genome sizes, using 5S and 45S rDNA genes as probes, the number of 5S rDNA loci was 4 in the common bean and stable among the common bean accessions, while the number of 45S rDNA loci was highly polymorphic, varying between 6 and 16. Consequently, it was determined in the present study that the genetic resource collection of common bean had a wide variation in terms of genome size and genome organization.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [116O057]
dc.description.sponsorshipWe would like to thank the Scientific and Technological Research Council of Turkey (TUBITAK) for providing financial support for this study under project no. 116O057.
dc.identifier.doi10.3906/tar-1910-33
dc.identifier.endpage630
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85097380947
dc.identifier.scopusqualityQ2
dc.identifier.startpage612
dc.identifier.urihttps://doi.org/10.3906/tar-1910-33
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10179
dc.identifier.volume44
dc.identifier.wosWOS:000599463300007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorYücel, Gülru
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherTubitak Scientific & Technical Research Council Turkey
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectPhaseolus vulgaris
dc.subjectgenome size
dc.subjectflow cytometry
dc.subjectgeographical variables
dc.subjectfluorescence in situ hybridization
dc.subjectgene pool
dc.subjectGenome Size Variation
dc.subjectNuclear-Dna Content
dc.subjectBrachypodium-Distachyon
dc.subjectNatural-Populations
dc.subjectGenetic Diversity
dc.subjectChromosome Identification
dc.subjectIntraspecific Variation
dc.subjectRibosomal Dna
dc.subjectLunatus L.
dc.subjectRdna Loci
dc.titleMolecular cytogenetic characterization of common bean (Phaseolus vulgaris L.) accessions
dc.typeArticle

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