The value of frozen cartilage tissues without cryoprotection for genetic conservation

dc.authorid0000-0002-2789-0635
dc.authorscopusid16400954400
dc.authorscopusid49863405700
dc.authorscopusid6603553213
dc.authorwosidarat, sezen/ABA-7370-2020
dc.contributor.authorÇetinkaya, Gaye
dc.contributor.authorHatipoğlu, İbrahim
dc.contributor.authorArat, Sezen
dc.date.accessioned2022-05-11T14:45:23Z
dc.date.available2022-05-11T14:45:23Z
dc.date.issued2014
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractAnimal tissues frozen without cryoprotection are thought to be inappropriate for use as a donor for somatic cell nuclear transfer (SCNT) studies. Cells in tissues that have been frozen without a cryoprotectant are commonly thought to be dead or to have lost genomic integrity. However, in this study we show that the frozen auricular cartilage tissues of anatolian buffalo contain a considerable number of viable healthy cells. The cells in auricular cartilage tissues are resistant to cryo-injury at -80 degrees C. Primary cell cultures were established from defrosted ear tissues which were frozen without cryoprotectant. The growth and functional characteristics of primary cell cultures are characterized according to cell growth curve, cell cycle analysis, karyotype and GAG synthesis. The results indicate that frozen cartilage tissues could be valuable materials for the conservation of species and SCNT technology. (C) 2013 Elsevier Inc. All rights reserved.
dc.description.sponsorshipTURHAYGEN-1 ProjectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [KAMAG-106G005]
dc.description.sponsorshipThis study was funded as a part of TURHAYGEN-1 Project (http://www.turkhaygen.gov.tr) by The Scientific and Technological Research Council of Turkey (Project No: KAMAG-106G005).
dc.identifier.doi10.1016/j.cryobiol.2013.11.008
dc.identifier.endpage70
dc.identifier.issn0011-2240
dc.identifier.issn1090-2392
dc.identifier.issue1en_US
dc.identifier.pmid24291088
dc.identifier.scopus2-s2.0-84896697984
dc.identifier.scopusqualityQ1
dc.identifier.startpage65
dc.identifier.urihttps://doi.org/10.1016/j.cryobiol.2013.11.008
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10029
dc.identifier.volume68
dc.identifier.wosWOS:000332190100009
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorArat, Sezen
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofCryobiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBiobanking
dc.subjectConservation
dc.subjectCryoinjury
dc.subjectCartilage
dc.subjectGene preservation
dc.subjectDirect Embryonic-Development
dc.subjectNuclear Transfer
dc.subjectSomatic-Cells
dc.subjectStorage Time
dc.subjectMouse Cells
dc.subjectIn-Vitro
dc.subjectCryopreservation
dc.subjectSpermatozoa
dc.subjectViability
dc.subjectBodies
dc.titleThe value of frozen cartilage tissues without cryoprotection for genetic conservation
dc.typeArticle

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