The value of frozen cartilage tissues without cryoprotection for genetic conservation
dc.authorid | 0000-0002-2789-0635 | |
dc.authorscopusid | 16400954400 | |
dc.authorscopusid | 49863405700 | |
dc.authorscopusid | 6603553213 | |
dc.authorwosid | arat, sezen/ABA-7370-2020 | |
dc.contributor.author | Çetinkaya, Gaye | |
dc.contributor.author | Hatipoğlu, İbrahim | |
dc.contributor.author | Arat, Sezen | |
dc.date.accessioned | 2022-05-11T14:45:23Z | |
dc.date.available | 2022-05-11T14:45:23Z | |
dc.date.issued | 2014 | |
dc.department | Fakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü | |
dc.description.abstract | Animal tissues frozen without cryoprotection are thought to be inappropriate for use as a donor for somatic cell nuclear transfer (SCNT) studies. Cells in tissues that have been frozen without a cryoprotectant are commonly thought to be dead or to have lost genomic integrity. However, in this study we show that the frozen auricular cartilage tissues of anatolian buffalo contain a considerable number of viable healthy cells. The cells in auricular cartilage tissues are resistant to cryo-injury at -80 degrees C. Primary cell cultures were established from defrosted ear tissues which were frozen without cryoprotectant. The growth and functional characteristics of primary cell cultures are characterized according to cell growth curve, cell cycle analysis, karyotype and GAG synthesis. The results indicate that frozen cartilage tissues could be valuable materials for the conservation of species and SCNT technology. (C) 2013 Elsevier Inc. All rights reserved. | |
dc.description.sponsorship | TURHAYGEN-1 ProjectTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [KAMAG-106G005] | |
dc.description.sponsorship | This study was funded as a part of TURHAYGEN-1 Project (http://www.turkhaygen.gov.tr) by The Scientific and Technological Research Council of Turkey (Project No: KAMAG-106G005). | |
dc.identifier.doi | 10.1016/j.cryobiol.2013.11.008 | |
dc.identifier.endpage | 70 | |
dc.identifier.issn | 0011-2240 | |
dc.identifier.issn | 1090-2392 | |
dc.identifier.issue | 1 | en_US |
dc.identifier.pmid | 24291088 | |
dc.identifier.scopus | 2-s2.0-84896697984 | |
dc.identifier.scopusquality | Q1 | |
dc.identifier.startpage | 65 | |
dc.identifier.uri | https://doi.org/10.1016/j.cryobiol.2013.11.008 | |
dc.identifier.uri | https://hdl.handle.net/20.500.11776/10029 | |
dc.identifier.volume | 68 | |
dc.identifier.wos | WOS:000332190100009 | |
dc.identifier.wosquality | Q2 | |
dc.indekslendigikaynak | Web of Science | |
dc.indekslendigikaynak | Scopus | |
dc.indekslendigikaynak | PubMed | |
dc.institutionauthor | Arat, Sezen | |
dc.language.iso | en | |
dc.publisher | Academic Press Inc Elsevier Science | |
dc.relation.ispartof | Cryobiology | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | Biobanking | |
dc.subject | Conservation | |
dc.subject | Cryoinjury | |
dc.subject | Cartilage | |
dc.subject | Gene preservation | |
dc.subject | Direct Embryonic-Development | |
dc.subject | Nuclear Transfer | |
dc.subject | Somatic-Cells | |
dc.subject | Storage Time | |
dc.subject | Mouse Cells | |
dc.subject | In-Vitro | |
dc.subject | Cryopreservation | |
dc.subject | Spermatozoa | |
dc.subject | Viability | |
dc.subject | Bodies | |
dc.title | The value of frozen cartilage tissues without cryoprotection for genetic conservation | |
dc.type | Article |
Dosyalar
Orijinal paket
1 - 1 / 1
Küçük Resim Yok
- İsim:
- 10029.pdf
- Boyut:
- 1.36 MB
- Biçim:
- Adobe Portable Document Format
- Açıklama:
- Tam Metin / Full Text