The value of frozen cartilage tissues without cryoprotection for genetic conservation
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Dosyalar
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Press Inc Elsevier Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Animal tissues frozen without cryoprotection are thought to be inappropriate for use as a donor for somatic cell nuclear transfer (SCNT) studies. Cells in tissues that have been frozen without a cryoprotectant are commonly thought to be dead or to have lost genomic integrity. However, in this study we show that the frozen auricular cartilage tissues of anatolian buffalo contain a considerable number of viable healthy cells. The cells in auricular cartilage tissues are resistant to cryo-injury at -80 degrees C. Primary cell cultures were established from defrosted ear tissues which were frozen without cryoprotectant. The growth and functional characteristics of primary cell cultures are characterized according to cell growth curve, cell cycle analysis, karyotype and GAG synthesis. The results indicate that frozen cartilage tissues could be valuable materials for the conservation of species and SCNT technology. (C) 2013 Elsevier Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Biobanking, Conservation, Cryoinjury, Cartilage, Gene preservation, Direct Embryonic-Development, Nuclear Transfer, Somatic-Cells, Storage Time, Mouse Cells, In-Vitro, Cryopreservation, Spermatozoa, Viability, Bodies
Kaynak
Cryobiology
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
68
Sayı
1
