Are biological agents toxic to human chondrocytes and osteocytes?
Yükleniyor...
Dosyalar
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bmc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Purpose: The aim of the present study is to investigate the effects of biological agents (BAs) on human chondrocytes and osteocytes in vitro. Methods: Primary cell cultures obtained from gonarthrosis patients were divided into four groups, two of which were designated as control cultures of chondrocyte and osteocyte, and the other two groups were exposed to BAs administered via the culture medium. Cultured cells were characterized by immunophenotyping. Before and after administration of the agents, the cultures were observed by inverted and environmental scanning electron microscopy (ESEM). The number of live cells and the proliferation rate were monitored by MTT assay. Results: Rituximab and adalimumab were the least toxic agents to chondrocytes, whereas adalimumab and etanercept were to osteocytes. Conclusion: During periods of intense active inflammation, the concentration of the preferred BAs after inhibition of inflammation needs to be emphasized when their effects on cartilage and bone tissue are considered at the cellular level if the clinical practice is to continue.
Açıklama
Anahtar Kelimeler
Biological agents, Chondrotoxicity, Osteotoxicity, Primary cell culture, Rheumatoid-Arthritis, Local-Anesthetics, Adalimumab, Abatacept, Tocilizumab, Infliximab, Rituximab, Efficacy, Anakinra, Diseases
Kaynak
Journal of Orthopaedic Surgery and Research
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
10
