Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm

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dc.authorid0000-0001-7707-2705
dc.authorid0000-0002-7678-3289
dc.authorwosidDuman, Muhammed/T-1697-2019
dc.contributor.authorÜstüner, Burcu
dc.contributor.authorAlçay, Selim
dc.contributor.authorGökçe, Elif
dc.contributor.authorYılmaz, M. Melih
dc.contributor.authorAktar, Ahmet
dc.contributor.authorHüraydın, Oğuzhan
dc.contributor.authorNur, Zekariya
dc.date.accessioned2022-05-11T14:05:14Z
dc.date.available2022-05-11T14:05:14Z
dc.date.issued2022
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Dölerme ve Suni Tohumlama Ana Bilim Dalı
dc.description.abstractThis study aimed to investigate the effect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. The highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P 0.05). There was no significant difference in the rate of post-thaw defective acrosomes when the other extender groups were compared with the control (P 0.05). The highest percentage of post-thaw DNA fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant different from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can be used successfully for freezing ram semen.
dc.identifier.doi10.9775/kvfd.2021.26855
dc.identifier.issn1300-6045
dc.identifier.scopus2-s2.0-85127943400
dc.identifier.scopusqualityQ3
dc.identifier.trdizinid529985
dc.identifier.urihttps://doi.org/10.9775/kvfd.2021.26855
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4927
dc.identifier.wosWOS:000771743800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorGökçe, Elif
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCryopreservation
dc.subjectLyophilized extender
dc.subjectRainbow trout seminal plasma
dc.subjectRam semen
dc.subjectEgg-Yolk
dc.subjectSoybean Lecithin
dc.subjectDna-Damage
dc.subjectSemen
dc.subjectSpermatozoa
dc.subjectFertility
dc.subjectConservation
dc.subjectIntegrity
dc.subjectUnfrozen
dc.subjectGlycerol
dc.titleLyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm
dc.typeArticle

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