Long Term Incubation Resilience of Post-Thaw Ram Semen Diluted with Lecithin-Based Extender Supplemented with Bovine Serum Albumin

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dc.authorid0000-0002-7678-3289
dc.authorscopusid56099810300
dc.authorscopusid56480349200
dc.authorscopusid56779799700
dc.authorscopusid55670728900
dc.authorscopusid18937724600
dc.authorscopusid6508060684
dc.authorwosidGokce, Elif/ABA-6294-2020
dc.authorwosidUstuner, Burcu/AAG-7238-2021
dc.authorwosidNur, Zekariya/AAH-2635-2021
dc.contributor.authorAlcay, Selim
dc.contributor.authorToker, Mehmed Berk
dc.contributor.authorGökçe, Elif
dc.contributor.authorÖnder, Nail Tekin
dc.contributor.authorÜstüner, Burcu
dc.contributor.authorNur, Zekariya
dc.date.accessioned2022-05-11T14:42:15Z
dc.date.available2022-05-11T14:42:15Z
dc.date.issued2019
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Bilimler Bölümü, Dölerme ve Suni Tohumlama Ana Bilim Dalı
dc.description.abstractThe objective of the study was to determine the optimum concentration of BSA in lecithin-based extender for post-thawing quality and incubation resilience (0 h, 6 h and 10 h) of ram sperm. Ejaculates were collected from five rams via electro ejaculation. Ejaculates were mixed to obtain pooled semen.Then, pooled semen was diluted with soybean lecithin-based extender without BSA (control) or supplemented with different concentrations of BSA (2.5 mg/mL, 5 mg/mL, 7.5 mg/mL and 10 mg/mL), at a final concentration of 150x106 spermatozoon/mL. Sperm motility, plasma membrane functional integrity (HOST), mitochondrial activity (rhodamine123), capacitation status (CTC), and DNA integrity (TUNEL) were evaluated. At the 10 h incubation, motility, plasma membrane functional integrity and mitochondria! function were better preserved in the BSA5 group compared to the control group. It was determined that high doses of BSA (5 mg/mL, 7.5 mg/mL and 10 mg/mL) affected acrosome reaction.The highest acrosome reaction rates were obtained in BSA10 groups in 6 h and 10 h incubation (P<0.05). TUNEL assay demonstrated that there were no differences among groups for DNA fragmentation at post-thaw and during incubation periods. The study shows that BSA supplemented extenders may have beneficial effect on ram semen parameters at 0 h, 6 h and 10 h of incubation. The results of the present study demonstrated a remarkable advantage of using 5 mg/mL of BSA in 1% lecithin-based extender.
dc.description.sponsorshipUludag University Scientific Research Projects Unit, Bursa, Turkey, (BAP)Uludag University [KUAP (V)-2015/55]
dc.description.sponsorshipThis work was supported by the Uludag University Scientific Research Projects Unit, Bursa, Turkey, (BAP) (Project number: KUAP (V)-2015/55
dc.identifier.doi10.9775/kvfd.2018.20843
dc.identifier.endpage297
dc.identifier.issn1300-6045
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85067432770
dc.identifier.scopusqualityQ3
dc.identifier.startpage291
dc.identifier.urihttps://doi.org/10.9775/kvfd.2018.20843
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9303
dc.identifier.volume25
dc.identifier.wosWOS:000460503100002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorGökçe, Elif
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisi
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBovine serum albumin
dc.subjectCryopreservation
dc.subjectIncubation resilience
dc.subjectRam spermatozoa
dc.subjectSoybean Lecithin
dc.subjectEgg-Yolk
dc.subjectSperm Capacitation
dc.subjectDna Fragmentation
dc.subjectLiquid Storage
dc.subjectQuality
dc.subjectAntioxidants
dc.subjectSpermatozoa
dc.subjectMotility
dc.subjectCryopreservation
dc.titleLong Term Incubation Resilience of Post-Thaw Ram Semen Diluted with Lecithin-Based Extender Supplemented with Bovine Serum Albumin
dc.title.alternativeSığır Serum Albumini İlave Edilmiş Lesitin Bazlı Sulandırıcı İle Sulandırılan Koç Spermasının Dondurma-Çözdürme Sonrası Uzun Süreli İnkübasyon Direnci]
dc.typeArticle

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