Assessment of Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey accessions using universal rice primers and molecular cytogenetics

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dc.authorscopusid6602664701
dc.authorscopusid57221307133
dc.authorscopusid57190797212
dc.authorscopusid57225437022
dc.contributor.authorTiryaki, İskender
dc.contributor.authorBaytekin Karaoğlu, Gülhan
dc.contributor.authorYücel, Gülru
dc.contributor.authorTuna, Metin
dc.date.accessioned2022-05-11T14:02:55Z
dc.date.available2022-05-11T14:02:55Z
dc.date.issued2021
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractThe aim of this study was to make morphological and molecular characterization of tall wheatgrass (Thinopyrum ponticum) accessions naturally found in Canakkale flora, Turkey. The seeds collected from 24 different locations in Canakkale vicinity were planted in the nursery field to determine thirteen morphological parameters. Twelve universal rice primers (URPs) were used to reveal genetic relationship among the accessions while ploidy analysis was done based on the nuclear DNA content of plants determined by Flow cytometer. Fluorescence in situ hybridization (FISH) method was used to determine 5S and 45S rDNA distributions on mitotic chromosomes. The agro-morphological data showed significant variation among the accessions for all parameters measured, except the number of nodes per plant. Twelve URP primers produced 73 alleles in total and 63 of those were polymorphic. The highest polymorphism information content value was obtained from URP 17R with 0.82. The first three components of Eigen values in PCA analysis explained 41.1% of total variation. The 2C nuclear DNA contents of the accessions ranged from 41.17 to 45.49 pg. All the accessions were determined as decaploid with 2n = 70 chromosomes. FISH analysis provided 18–20 interstial 5S rDNA and 12–14 terminal 45S rDNA loci. The results concluded that tall wheatgrass accessions used in this study contain a significant variation in morphological traits and PCR-based DNA polymorphism which could be used as a new genetic resource in breeding programs of tall wheatgrass and wheat improvement for intra- and intergeneric crosses. © 2021, The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature.
dc.description.sponsorshipÇanakkale Onsekiz Mart Üniversitesi, ÇOMÜ; British Association for Psychopharmacology, BAP: FYL-2017-1085
dc.description.sponsorshipThe financial support of this study was provided by Canakkale Onsekiz Mart University (COMU, BAP Grant #: FYL-2017-1085). We thank Ersin Karabacak for his help on taxonomic verification.
dc.identifier.doi10.1007/s10722-020-01101-4
dc.identifier.endpage1888
dc.identifier.issn0925-9864
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85098771326
dc.identifier.scopusqualityQ2
dc.identifier.startpage1875
dc.identifier.urihttps://doi.org/10.1007/s10722-020-01101-4
dc.identifier.urihttps://hdl.handle.net/20.500.11776/4540
dc.identifier.volume68
dc.identifier.wosWOS:000604808400009
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorYücel, Gülru
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherSpringer Science and Business Media B.V.
dc.relation.ispartofGenetic Resources and Crop Evolution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDNA marker
dc.subjectFISH
dc.subjectFlow cytometer
dc.subjectrDNA
dc.subjectTall wheatgrass
dc.subjectURPs
dc.subjectassessment method
dc.subjectcytogenetics
dc.subjectcytology
dc.subjectDNA
dc.subjectgenetic analysis
dc.subjectgenetic resource
dc.subjecthybridization
dc.subjectmolecular analysis
dc.subjectmorphology
dc.subjectCanakkale [Turkey]
dc.subjectTurkey
dc.subjectLophopyrum elongatum
dc.subjectMeleagris gallopavo
dc.subjectThinopyrum ponticum
dc.titleAssessment of Thinopyrum ponticum (Podp.) Barkworth & D. R. Dewey accessions using universal rice primers and molecular cytogenetics
dc.typeArticle

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