Development and validation of a sensitive spectrofluorimetric method for the determination of cilazapril of human plasma, urine, in pure and pharmaceutical preparations
Yükleniyor...
Dosyalar
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Maik Nauka/Interperiodica/Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A selective and sensitive spectrofluorimetric method was developed and validated for the determination of cilazapril in human plasma urine, in pure and pharmaceutical preparations. The proposed method is based on derivatization using 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl chloride) as fluorogenic agent and measuring the fluorescence of the products at emission wavelengths of 503 nm after excitation at 374 nm. The method was validated for linearity, limit of detection, limit of quantification, precision, accuracy, recovery. The calibration curves were linear over a concentration range of 100-500 and 50-250 ng/mL for plasma and urine, respectively. The limits of detection were calculated to be 0.26 and 31.59 ng/mL for plasma and urine, respectively. The proposed method was applied to study of cilazapril in pure, human plasma, urine, and pharmaceutical preparations.
Açıklama
Anahtar Kelimeler
Performance Liquid-Chromatography, Active Metabolite Cilazaprilat, Enzyme-Inhibitor Cilazapril, Quantitative-Determination, Hydrochlorothiazide, Formulations
Kaynak
Optics and Spectroscopy
WoS Q Değeri
Q4
Scopus Q Değeri
Q4
Cilt
119
Sayı
2
