Development and validation of a sensitive spectrofluorimetric method for the determination of cilazapril of human plasma, urine, in pure and pharmaceutical preparations
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Date
2015
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Maik Nauka/Interperiodica/Springer
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
A selective and sensitive spectrofluorimetric method was developed and validated for the determination of cilazapril in human plasma urine, in pure and pharmaceutical preparations. The proposed method is based on derivatization using 1-dimethylaminonaphthalene-5-sulphonyl chloride (dansyl chloride) as fluorogenic agent and measuring the fluorescence of the products at emission wavelengths of 503 nm after excitation at 374 nm. The method was validated for linearity, limit of detection, limit of quantification, precision, accuracy, recovery. The calibration curves were linear over a concentration range of 100-500 and 50-250 ng/mL for plasma and urine, respectively. The limits of detection were calculated to be 0.26 and 31.59 ng/mL for plasma and urine, respectively. The proposed method was applied to study of cilazapril in pure, human plasma, urine, and pharmaceutical preparations.
Description
Keywords
Performance Liquid-Chromatography, Active Metabolite Cilazaprilat, Enzyme-Inhibitor Cilazapril, Quantitative-Determination, Hydrochlorothiazide, Formulations
Journal or Series
Optics and Spectroscopy
WoS Q Value
Q4
Scopus Q Value
Q4
Volume
119
Issue
2
