Cryptosporidium parvum oocyst viability and behaviour of the residual body during the excystation process
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Date
2011
Journal Title
Journal ISSN
Volume Title
Publisher
Springer
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
This study was conducted as a comparative evaluation of time-dependent changes in the viability of purified Cryptosporidium parvum oocysts by means of different excystation methods. Oocyst samples were 2 weeks to 12 months old and were treated with bile or sodium taurocholate, partly after pretreatment with hypochlorite. Pretreatment markedly enhanced the excystation of younger oocyst samples but did not increase excystation rates of 9 or 12-month-old oocysts. A cell culture-PCR assay was used as a second indicator for oocyst viability and was most consistent with excystation trials including oocyst pretreatment. In experiments aiming at the determination of the behaviour of the oocyst residual body during excystation, it could be demonstrated that it might be involved in this process.
Description
Keywords
In-Vitro Excystation
Journal or Series
Parasitology Research
WoS Q Value
Q2
Scopus Q Value
Q1
Volume
109
Issue
6
