Determination of intraspecific nuclear DNA content variation in common vetch (Vicia sativa L.) lines and cultivars based on two distinct internal reference standards

Basit Öğe Kaydı
dc.authorscopusid6602664701
dc.authorscopusid57225437022
dc.authorwosidTuna, Metin/ABA-4295-2020
dc.contributor.authorTiryaki, İskender
dc.contributor.authorTuna, Metin
dc.date.accessioned2022-05-11T14:45:46Z
dc.date.available2022-05-11T14:45:46Z
dc.date.issued2012
dc.departmentFakülteler, Ziraat Fakültesi, Tarla Bitkileri Bölümü
dc.description.abstractThe 2C nuclear DNA contents of 40 common vetch (Vicia sativa L.) lines and cultivars were determined by using both safflower (Carthamus tinctorius L.) and barley (Hordeum vulgare L.) as internal reference standards in flow cytometry analysis and correlated with plant morphological characters and 1000-seed weight. The t-test results showed a significant difference between the mean DNA values of the barley and safflower standards (t = -16.74, P = 0.0001). Data analysis also indicated significant (P < 0.01) intraspecific nuclear DNA content variation within the common vetch lines and cultivars for both internal standards used. The DNA content values ranged from 3.342 pg to 3.652 pg and from 3.600 pg to 4.002 pg for the internal standards of safflower and barley, respectively. The DNA differences within the internal standards were 0.310 pg and 0.402 pg for safflower and barley, respectively. The internal standard of barley constantly produced higher DNA content values than the values of safflower standard for all vetch lines and cultivars. Nuclear DNA content differences between 2 internal standards for the same plant material reached as high as 15.59% (0.540 pg), which is equivalent to 528.12 Mbp DNA. No significant correlations between 1000-seed weight and the nuclear DNA contents of barley (P < 0.51) or safflower (P < 0.76) were detected.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TOVAG-107O012]
dc.description.sponsorshipFinancial support of this work was provided by the Scientific and Technological Research Council of Turkey (TUBITAK) with research grant TOVAG-107O012. The efforts of Abdullah Cil, who provided most of the starter plant material, are also greatly appreciated.
dc.identifier.doi10.3906/tar-1201-47
dc.identifier.endpage653
dc.identifier.issn1300-011X
dc.identifier.issn1303-6173
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-84868030920
dc.identifier.scopusqualityQ2
dc.identifier.startpage645
dc.identifier.urihttps://doi.org/10.3906/tar-1201-47
dc.identifier.urihttps://hdl.handle.net/20.500.11776/10143
dc.identifier.volume36
dc.identifier.wosWOS:000312423900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorTuna, Metin
dc.language.isoen
dc.publisherTubitak Scientific & Technical Research Council Turkey
dc.relation.ispartofTurkish Journal of Agriculture and Forestry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDNA C-value
dc.subjectflow cytometry
dc.subjectintraspecific variation
dc.subjectnuclear genome size
dc.subjectnuclear DNA amount
dc.subjectGenome Size Variation
dc.subjectFlow-Cytometric Analysis
dc.subjectChromosome-Number
dc.subjectFestuca-Pallens
dc.subjectMaturity Group
dc.subjectEvolution
dc.subjectPopulations
dc.subjectDifferentiation
dc.subjectHybridization
dc.subjectConsequences
dc.titleDetermination of intraspecific nuclear DNA content variation in common vetch (Vicia sativa L.) lines and cultivars based on two distinct internal reference standards
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
10143.pdf
Boyut:
108.23 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin / Full Text
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu
Ziraat Fakültesi Koleksiyonu