A New Approach to DNA Isolation from Dileptid Ciliates (Ciliophora: Litostomatea: Rhynchostomatia)

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dc.authorwosidŞenler, Naciye Gülkız/ABA-3358-2020
dc.contributor.authorUral, Hilal
dc.contributor.authorBircan, Rifat
dc.contributor.authorAral, Cenk
dc.contributor.authorYıldız, İsmail
dc.contributor.authorŞenler, Naciye Gülkız
dc.date.accessioned2022-05-11T14:14:30Z
dc.date.available2022-05-11T14:14:30Z
dc.date.issued2020
dc.departmentFakülteler, Fen Edebiyat Fakültesi, Biyoloji Bölümü
dc.departmentFakülteler, Ziraat Fakültesi, Zootekni Bölümü
dc.description.abstractMolecular diagnostic methods have been used to supplement morphological methods in taxonomy. In this study, we used molecular methods to diagnose populations of dileptid ciliates, which commonly occur in terrestrial and semi-terrestrial habitats. Molecular investigations based on the comparison of DNA sequences have been carried out with a limited number of ciliate species due to insufficient genomic DNA for PCR because of their small population size and difficulties to maintain monocultures. The present study shows that there is a loss of DNA with conventional methods and proposes a novel approach: cells (stored at -20 degrees C or fresh) are directly subjected to PCR, without being processed by any chemical treatment. The small subunit ribosomal DNA (SSU rDNA) (18S rRNA gene) of dileptid ciliates was successfully amplified by direct PCR, without DNA extraction, for single-cell and multi-cell samples. This method has been found to be simpler and especially useful for species that are rare and have small population sizes.
dc.description.sponsorshipNamik Kemal University Scientific Research Projects Coordination UnitNamik Kemal University [NKUBAP.01, 16.079]
dc.description.sponsorshipThis work was supported by the Namik Kemal University Scientific Research Projects Coordination Unit (Project number: NKUBAP.01.GA.16.079). We are grateful to Dr. Deniz Sirin for his valuable comments in data analysis. We thank also Dr. Elife Zerrin Bagci for significantly improving the language of manuscript.
dc.identifier.endpage205
dc.identifier.issn0324-0770
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85101572411
dc.identifier.scopusqualityQ4
dc.identifier.startpage199
dc.identifier.urihttps://hdl.handle.net/20.500.11776/5919
dc.identifier.volume72
dc.identifier.wosWOS:000542628500005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorUral, Hilal
dc.institutionauthorBircan, Rifat
dc.institutionauthorAral, Cenk
dc.institutionauthorŞenler, Naciye Gülkız
dc.language.isoen
dc.publisherInst Zoology, Bas
dc.relation.ispartofActa Zoologica Bulgarica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectDNA extraction
dc.subjectdirect PCR
dc.subjectSSU rDNA
dc.subjectCiliophora
dc.subjectdileptid ciliates
dc.subjectExtracting Genomic Dna
dc.subjectMolecular Characterization
dc.subjectSpec. Nov
dc.subjectDiversity
dc.subjectProtista
dc.subjectProtozoa
dc.titleA New Approach to DNA Isolation from Dileptid Ciliates (Ciliophora: Litostomatea: Rhynchostomatia)
dc.typeArticle

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