Engineering of xylanases for the development of biotechnologically important characteristics

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dc.authoridSURMELI, YUSUF/0000-0002-9645-6314
dc.contributor.authorSürmeli, Yusuf
dc.contributor.authorSanli-Mohamed, Gülşah
dc.date.accessioned2023-05-06T17:23:41Z
dc.date.available2023-05-06T17:23:41Z
dc.date.issued2023
dc.departmentFakülteler, Ziraat Fakültesi, Tarımsal Biyoteknoloji Bölümü
dc.description.abstractXylanases are the main biocatalysts used for the reduction of the xylan backbone from hemicellulose, randomly splitting off beta-1,4-glycosidic linkages between xylopyranosyl residues. Xylanase market has been annually estimated at 500 million US Dollars and they are potentially used in broad industrial process ranges such as paper pulp biobleaching, xylo-oligosaccharide production, and biofuel manufacture from lignocellulose. The highly stable xylanases are preferred in the downstream procedure of industrial processes because they can tolerate severe conditions. Almost all native xylanases can not endure adverse conditions thus they are industrially not proper to be utilized. Protein engineering is a powerful technology for developing xylanases, which can effectively work in adverse conditions and can meet requirements for industrial processes. This study considered state-of-the-art strategies of protein engineering for creating the xylanase gene diversity, high-throughput screening systems toward upgraded traits of the xylanases, and the prediction and comprehensive analysis of the target mutations in xylanases by in silico methods. Also, key molecular factors have been elucidated for industrial characteristics (alkaliphilic enhancement, thermal stability, and catalytic performance) of GH11 family xylanases. The present review explores industrial characteristics improved by directed evolution, rational design, and semi-rational design as protein engineering approaches for pulp bleaching process, xylooligosaccharides production, and biorefinery & bioenergy production.
dc.identifier.doi10.1002/bit.28339
dc.identifier.endpage1188
dc.identifier.issn0006-3592
dc.identifier.issn1097-0290
dc.identifier.issue5en_US
dc.identifier.pmid36715367
dc.identifier.scopus2-s2.0-85147493474
dc.identifier.scopusqualityQ1
dc.identifier.startpage1171
dc.identifier.urihttps://doi.org/10.1002/bit.28339
dc.identifier.urihttps://hdl.handle.net/20.500.11776/12242
dc.identifier.volume120
dc.identifier.wosWOS:000928493700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorSürmeli, Yusuf
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology And Bioengineering
dc.relation.publicationcategoryDiğeren_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectbiorefinery & bioenergy
dc.subjectengineering xylanase
dc.subjectindustrial characteristics
dc.subjectprotein engineering approaches
dc.subjectpulp bleaching
dc.subjectstate-of-the-art strategies
dc.subjectXOs production
dc.subjectSite-Directed Mutagenesis
dc.subjectCellulase-Free Xylanase
dc.subjectFamily 11 Xylanase
dc.subjectGh11 Xylanase
dc.subjectCatalytic Performance
dc.subjectGeobacillus-Stearothermophilus
dc.subjectThermostability Improvement
dc.subjectBioethanol Production
dc.subjectEnzymatic-Hydrolysis
dc.subjectThermal Performance
dc.titleEngineering of xylanases for the development of biotechnologically important characteristics
dc.typeReview Article

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