Engineering of xylanases for the development of biotechnologically important characteristics
Küçük Resim Yok
Tarih
2023
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Xylanases are the main biocatalysts used for the reduction of the xylan backbone from hemicellulose, randomly splitting off beta-1,4-glycosidic linkages between xylopyranosyl residues. Xylanase market has been annually estimated at 500 million US Dollars and they are potentially used in broad industrial process ranges such as paper pulp biobleaching, xylo-oligosaccharide production, and biofuel manufacture from lignocellulose. The highly stable xylanases are preferred in the downstream procedure of industrial processes because they can tolerate severe conditions. Almost all native xylanases can not endure adverse conditions thus they are industrially not proper to be utilized. Protein engineering is a powerful technology for developing xylanases, which can effectively work in adverse conditions and can meet requirements for industrial processes. This study considered state-of-the-art strategies of protein engineering for creating the xylanase gene diversity, high-throughput screening systems toward upgraded traits of the xylanases, and the prediction and comprehensive analysis of the target mutations in xylanases by in silico methods. Also, key molecular factors have been elucidated for industrial characteristics (alkaliphilic enhancement, thermal stability, and catalytic performance) of GH11 family xylanases. The present review explores industrial characteristics improved by directed evolution, rational design, and semi-rational design as protein engineering approaches for pulp bleaching process, xylooligosaccharides production, and biorefinery & bioenergy production.
Açıklama
Anahtar Kelimeler
biorefinery & bioenergy, engineering xylanase, industrial characteristics, protein engineering approaches, pulp bleaching, state-of-the-art strategies, XOs production, Site-Directed Mutagenesis, Cellulase-Free Xylanase, Family 11 Xylanase, Gh11 Xylanase, Catalytic Performance, Geobacillus-Stearothermophilus, Thermostability Improvement, Bioethanol Production, Enzymatic-Hydrolysis, Thermal Performance
Kaynak
Biotechnology And Bioengineering
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
120
Sayı
5
