Expression and production of recombinant proteins from immunodominant E gene regions of bovine viral diarrhoea virus 1 (BVDV-1) Turkish field strains for prophylactic purpose

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dc.authorid0000-0003-4021-2414
dc.authorid0000-0002-4279-6233
dc.authorid0000-0003-4021-2414
dc.authorscopusid6506646891
dc.authorscopusid23028324100
dc.authorscopusid36130568600
dc.authorscopusid55921283100
dc.authorscopusid56190454300
dc.authorscopusid6701740969
dc.authorscopusid6603549695
dc.authorwosidOguzoglu, Tuba Cigdem/ABF-3972-2021
dc.authorwosidKOÇ, B. Taylan/W-5112-2019
dc.authorwosidMuz, Dilek pinar/AAR-8673-2020
dc.authorwosidOguzoglu, Tuba Cigdem/V-5130-2018
dc.authorwosidTimurkan, Mehmet Ozkan/AAQ-4934-2021
dc.contributor.authorOğuzoğlu, T. C.
dc.contributor.authorMuz, Dilek
dc.contributor.authorTimurkan, M. O.
dc.contributor.authorKoç, B. T.
dc.contributor.authorÖzşahin, E.
dc.contributor.authorBurgu, I.
dc.contributor.authorDemirbağ, Z.
dc.date.accessioned2022-05-11T14:42:21Z
dc.date.available2022-05-11T14:42:21Z
dc.date.issued2017
dc.departmentFakülteler, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Viroloji Ana Bilim Dalı
dc.description.abstractSince the first description of Bovine Viral Diarrhoea Virus (BVDV) infection, many studies have been performed, and control and eradication programs were developed worldwide. Several studies have been conducted on the presence/prevalence and molecular characterization of BVD viruses in Turkey. However, there is no effective struggle programs for BVDV infection up to now in Turkey. Current study has been conducted for the identification of the E gene region which encodes several immunogenic proteins of BVDV, tested the immunogenicity of the recombinant proteins for possible vaccine production. We focused on the molecular characterization of the E gene region of field pestivirus isolates (n=40) which were obtained in Turkey between 1997 and 2007. Two recombinant proteins, E-ms and E2, were chosen from the E region and expressed in baculovirus vector system. The acquired proteins (approximately 34 kDa for E-ms belonging to BVDV 1-a and 44 kDa for E2 belonging to BVDV 1-l) triggered a humoral immune response in mice. These proteins could represent a good starting material in preparation of vaccines which are indispensable for control and eradication programs of pestivirus infections in Turkey.
dc.description.sponsorshipTUBITAK TOVAG,TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110O915]
dc.description.sponsorshipAnimal experiments were conducted follow in the approval of Ankara University Animal Experiments Local Ethical Commitee (Number: 2010-96, 2010-292, 201096-335). This study was supported by TUBITAK TOVAG 110O915, Turkey. The authors thank Alexander Postel for the critisims of designing of primers in this study.
dc.identifier.endpage191
dc.identifier.issn0035-1555
dc.identifier.issn2258-0646
dc.identifier.issue45542en_US
dc.identifier.scopus2-s2.0-85027458734
dc.identifier.scopusqualityQ3
dc.identifier.startpage183
dc.identifier.urihttps://hdl.handle.net/20.500.11776/9346
dc.identifier.volume168
dc.identifier.wosWOS:000408492200006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorMuz, Dilek
dc.language.isoen
dc.publisherEcole Nationale Veterinaire Toulouse
dc.relation.ispartofRevue De Medecine Veterinaire
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBVDV
dc.subjectvaccine candidate recombinant proteins
dc.subjectE-ms
dc.subjectE2
dc.subjectTurkey
dc.subjectGlycoprotein E2
dc.subjectMolecular Characterization
dc.subjectImmune-Responses
dc.subjectCattle
dc.subjectIdentification
dc.subjectPestiviruses
dc.subjectVaccination
dc.subjectAntibodies
dc.subjectDiversity
dc.subjectFrequency
dc.titleExpression and production of recombinant proteins from immunodominant E gene regions of bovine viral diarrhoea virus 1 (BVDV-1) Turkish field strains for prophylactic purpose
dc.typeArticle

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