Expression and production of recombinant proteins from immunodominant E gene regions of bovine viral diarrhoea virus 1 (BVDV-1) Turkish field strains for prophylactic purpose
Yükleniyor...
Dosyalar
Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Ecole Nationale Veterinaire Toulouse
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Since the first description of Bovine Viral Diarrhoea Virus (BVDV) infection, many studies have been performed, and control and eradication programs were developed worldwide. Several studies have been conducted on the presence/prevalence and molecular characterization of BVD viruses in Turkey. However, there is no effective struggle programs for BVDV infection up to now in Turkey. Current study has been conducted for the identification of the E gene region which encodes several immunogenic proteins of BVDV, tested the immunogenicity of the recombinant proteins for possible vaccine production. We focused on the molecular characterization of the E gene region of field pestivirus isolates (n=40) which were obtained in Turkey between 1997 and 2007. Two recombinant proteins, E-ms and E2, were chosen from the E region and expressed in baculovirus vector system. The acquired proteins (approximately 34 kDa for E-ms belonging to BVDV 1-a and 44 kDa for E2 belonging to BVDV 1-l) triggered a humoral immune response in mice. These proteins could represent a good starting material in preparation of vaccines which are indispensable for control and eradication programs of pestivirus infections in Turkey.
Açıklama
Anahtar Kelimeler
BVDV, vaccine candidate recombinant proteins, E-ms, E2, Turkey, Glycoprotein E2, Molecular Characterization, Immune-Responses, Cattle, Identification, Pestiviruses, Vaccination, Antibodies, Diversity, Frequency
Kaynak
Revue De Medecine Veterinaire
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
168
Sayı
45542
