The Identification of Novel Single-Nucleotide Polymorphisms of Equine Beta-Lactoglobulin and Lactotransferrin Genes
Yükleniyor...
Dosyalar
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
W.B. Saunders
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Lactoferrin (LTF), also called lactotransferrin, is an iron-binding protein and member of transferrin family, whereas ?-LG is an important milk protein and belongs to the ligand-binding protein family of lipocalins and binds retinol. Beta-lactoglobulin (?-LG) is a major whey protein and it is a member of lipocalins family. ?-LG is also known as a major allergen reason of cow milk protein allergy. Yet, no information has been reported about ?-LG and LTF genes in donkeys. In this study, the genetic variation of ?-LG and LTF genes were analyzed via DNA sequencing in 77 donkeys, which were individuals reared in Thrace region of Turkey. Four novel SNPs are identified as g.12887G>A, g.12973G>A, g.12654G>A, and g.13057T>C in the third intron region of ?-LG gene and a genetic variant g.272719G>A in the 14th intron of LTF gene. The DNA sequences of ?-LG and LTF genes in donkeys are reported for the first time in this study and these sequences were deposited to NCBI GenBank database, with the accession number MK125015 and MK211165, respectively. These variations may have an effect on milk yield and content in donkeys, so further investigations are needed to prove this hypothesis and these may be candidate SNPs for donkey breeding. © 2019 Elsevier Inc.
Açıklama
Anahtar Kelimeler
DNA sequencing, Donkey, Equus asinus, LTF gene, ?-LG gene, beta lactoglobulin, lactoferrin, lactoferrin, lactoglobulin, nucleotide, Article, DNA sequence, donkey, gene identification, genetic variability, genetic variation, intron, milk yield, nonhuman, nucleotide sequence, single nucleotide polymorphism, Turkey (republic), animal, bovine, Equidae, female, genetics, horse, turkey (bird), Animals, Cattle, Equidae, Female, Horses, Lactoferrin, Lactoglobulins, Nucleotides, Polymorphism, Single Nucleotide, Turkey
Kaynak
Journal of Equine Veterinary Science
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
75
